Preparative Isolation and Purification of Saponin and Flavone Glycoside Compounds from Clinopodium chinensis (Benth) O. Kuntze by High-Speed Countercurrent Chromatography

• Published in: Journal of liquid chromatography & related technologies Vol. 30; no. 4; pp. 521 - 532
• Main Authors: Liu, Renmin, Kong, Lingyi, Li, Aifeng, Sun, Ailing
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 01.03.2007; Taylor & Francis
• Subjects: Biological and medical sciences; Clinopodiside A; Clinopodium chinensis (Benth) O. Kuntze; Didymin; General pharmacology; HSCCC; Medical sciences; Nairutin; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Asia; China; Countercurrent chromatography; Clinopodiside A; Nairutin; Purification; Pharmacognosy; HSCCC; Preparative chromatography; Flavonoid; Flavanone derivatives; Medicinal plant; Saponin; Oleanane derivatives; Clinopodium chinensis (Benth) O. Kuntze; Folk medicine; Labiatae; Dicotyledones; Didymin; Angiospermae; Plant origin; Flavone derivatives; Isolation; Spermatophyta; Glycoside
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1080/10826070601093846

A preparative high-speed countercurrent chromatography (HSCCC) method for isolation and purification of didymin, nairutin, and clinopodiside A from Clinopodium chinensis (Benth) O. Kuntze (Duanxueliu in Chinese) was successfully established. The separation was performed in two steps with two different kinds of solvent systems. In the first step, ethyl acetate-1-butanol-water (5:0.8:5, v/v) was used as the two-phase solvent system; nairutin was purified, and didymin and clinopodiside A were eluted together. In the second step, ethyl acetate-methanol-water (5:1:5, v/v) was used as the two-phase solvent system; didymin and clinopodiside A were separated and purified. After two-step separation, 15.2 mg of nairutin, 39.1 mg of clinopodiside A, and 20.6 mg of didymin were obtained from 100 mg of crude extract with purities of 96.5%, 98.4%, and 99.1%, respectively, as determined by HPLC analysis. The chemical structures of the three components were confirmed by 1 H NMR and 13 C NMR.