Cloning and analysis of the dnaG gene encoding Pseudomonas putida DNA primase

The dnaG gene coding for primase, a key enzyme in DNA replication, has been isolated from chromosomal DNA of the soil bacterium Pseudomonas putida. It maps within the putative MMS operon, between the rpsU and rpoD genes. Comparison of the deduced amino acid sequence of P. putida DnaG with sequences...

Full description

Saved in:
Bibliographic Details
Published inBiochimica et biophysica acta Vol. 1352; no. 3; pp. 243 - 248
Main Authors Szafranski, Przemyslaw, Smith, Cassandra L, Cantor, Charles R
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 26.06.1997
Subjects
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA Primase
DNA replication
DNA Replication - genetics
Genes, Bacterial
Molecular Sequence Data
Primase
Protein Structure, Secondary
Pseudomonas
Pseudomonas putida - genetics
RNA Nucleotidyltransferases - chemistry
RNA Nucleotidyltransferases - genetics
RNA, Messenger - chemistry
Sequence Alignment
Primase
Pseudomonas
DNA replication
Online AccessGet full text

Cover

More Information
Summary:The dnaG gene coding for primase, a key enzyme in DNA replication, has been isolated from chromosomal DNA of the soil bacterium Pseudomonas putida. It maps within the putative MMS operon, between the rpsU and rpoD genes. Comparison of the deduced amino acid sequence of P. putida DnaG with sequences of other known bacterial primases reveals the presence of a possible regulatory region which would be unique to pseudomonads. The analysis of nucleotide sequence suggests that stable folding of the dnaG mRNA may significantly contribute to the low level of its expression within a cell.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0167-4781
0006-3002
1879-2634
DOI:10.1016/S0167-4781(97)00059-6