Structural Studies of Expressed tIK, Anti-Inflammatory Peptide

Cytokine imbalance is one of the causes of inflammation. Inflammation has yet to be adequately treated without side effects. Therefore, we tried to develop a peptide drug with minimal side effects. Peptide drugs have the advantage of being bio-friendly and bio-specific. In a previous study, three pe...

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Bibliographic Details
Published inInternational journal of molecular sciences Vol. 24; no. 1; p. 636
Main Authors Kim, Minseon, Kim, Yongae
Format Journal Article
LanguageEnglish
Published Switzerland MDPI 30.12.2022
MDPI AG
Subjects
Anti-Inflammatory Agents - pharmacology
anti-inflammatory peptide
autoimmune diseases
Circular Dichroism
Cytokines - metabolism
expression
Humans
Inflammation
isolation
Peptides - chemistry
Peptides - pharmacology
Protein Structure, Secondary
rheumatoid arthritis
tIK
autoimmune diseases
expression
purification
tIK
anti-inflammatory peptide
isolation
rheumatoid arthritis
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Summary:Cytokine imbalance is one of the causes of inflammation. Inflammation has yet to be adequately treated without side effects. Therefore, we tried to develop a peptide drug with minimal side effects. Peptide drugs have the advantage of being bio-friendly and bio-specific. In a previous study, three peptides with anti-inflammatory activity were derived based on a truncated IK (tIK) protein, which was a fragment of the IK protein with anti-inflammatory effects. The objective of this study was to optimize the process of expressing, isolating, and purifying the three peptides using bacterial strains and describe the process. Circular dichroism and solution state nuclear magnetic resonance spectroscopy were performed on the final purified high-purity peptide and its secondary structure was also identified.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24010636