Induction of angiotensin converting enzyme in human monocytes in culture

Angiotensin converting enzyme (E.C.3.4.15.1, peptidyl dipeptidase) in circulating human monocytes rose from undetectable or minimal levels in vivo to as high as 35.5 nmol/min·mgprotein (>300-fold increase) after 6 or 7 days in culture. Enzyme induction was enhanced by autologous serum and exposur...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 83; no. 3; pp. 843 - 849
Main Authors Friedland, Joan, Setton, Charlotte, Silverstein, Emanuel
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 14.08.1978
Subjects
Cells, Cultured
Enzyme Induction
Humans
Lymphocytes - enzymology
Monocytes - enzymology
Peptidyl-Dipeptidase A - biosynthesis
PBSD
ACE
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Summary:Angiotensin converting enzyme (E.C.3.4.15.1, peptidyl dipeptidase) in circulating human monocytes rose from undetectable or minimal levels in vivo to as high as 35.5 nmol/min·mgprotein (>300-fold increase) after 6 or 7 days in culture. Enzyme induction was enhanced by autologous serum and exposure for two days to 0.45 μM dexamethasone. Potent inhibition of enzyme induction by 370 μg/ml of actinomycin D and 1 μM cycloheximide suggested that new messenger RNA and enzyme biosynthesis are involved in the induction. Human monocyte and lung enzyme were similar with respect to EDTA inhibition, CoCl 2 activation and inhibition by an antienzyme antiserum. Human lymphocytes had minimal or undetectable enzyme which was not induced after 4 days in culture.
Bibliography:ObjectType-Article-1
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ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(78)91471-7