Assessment of Biochemical and Antioxidative Status in Patients Suffering from Dengue Fever

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 35; no. 3; pp. 411 - 418
• Main Authors: Rasool, Mahmood, Malik, Arif, Khan, Khalid Mahmud, Qureshi, Muhammad Saeed, Shabbir, Beenish, Zahid, Sara, Asif, Muhammad, Manan, Abdul, Rashid, Sana, Khan, Saima Rubab, Arsalan, Hafiz Muhammad, Alam, Rabail, Arooj, Mahwish, Qazi, Mahmood Husain, Chaudhary, Adeel Gulzar Ahmed, Abuzenadah, Adel Mohammed, Al-Qahtani, Mohammed Hussain, Karim, Sajjad
• Format: Journal Article
• Language: English
• Published: Wuhan Huazhong University of Science and Technology 01.06.2015; Center of Excellence in Genomic Medicine Research, Faculty of Applied Medical Sciences,King Abdulaziz University, Jeddah 21589, Saudi Arabia%Institute of Molecular Biology and Biotechnology, the University of Lahore,Lahore 54000, Pakistan%Fatima Jinnah Medical College, Sir Gangaram Hospital Lahore, Lahore 54000, Pakistan%Department of Biotechnology and Informatics, BUITEMS, Quetta 87300, Pakistan
• Subjects: Adult; Antioxidants - metabolism; Biomarkers - blood; China; Dengue - classification; Dengue - diagnosis; Dengue - metabolism; Diagnosis, Differential; ELISA试验; Female; Humans; Immunoglobulin M - metabolism; Male; Medicine; Medicine & Public Health; Middle Aged; Young Adult; 丙氨酸氨基转移酶; 患者; 氧化状态; 生理生化指标; 登革热; 评估; 还原型谷胱甘肽; China; alanine amino transferase; aspartate amino transferase; dengue fever; malondialdehyde; superoxide dismutase; catalase
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-015-1446-x

Summary：A multi-centred study was designed to collect dengue epidemiologic data from government and registered private hospitals/clinics and maintained archive of frozen specimens in bio-bank to be used for future dengue epidemic control program, and assess the epidemiology of dengue fever（DF） by evaluating biochemical and oxidative status of patients. ELISA Ig M antibodies test was done to confirm DF. From August 2010 to December 2011, 101 confirmed blood samples of DF patients referred to pathology lab of Jinnah Hospital Lahore were subjected to the epidemiologic assessment by evaluating the biochemical and physiological indices and alterations of circulating antioxidants. Clinical features of DF patients and effect of fever on blood components and serum proteins of liver were recorded. The hospital stay in DF, dengue hemorrhagic fever（DHF） and dengue shock syndrome（DSS） showed significant difference. Significant increases in serum alanine amino transferase（ALT）（P=0.000）, aspartate amino transferase（AST）（P=0.000）, alkaline phosphatase（ALP）（P=0.000）, malondialdehyde（MDA） along with significant decreases in total protein（TP）（P=0.000）, reduced glutathione（GSH）（P=0.000）, superoxide dismutase（SOD）, catalase（CAT）（P=0.000）, and sialic acid contents（P=0.016） were observed. A positive correlation existed between bound sialic acid levels, liver enzymes and circulating antioxidants（r=0.656, P=0.016）. In the present study, alterations of circulating antioxidants in DF suggest that DF might be a metabolic response to an acute, self-limiting tropical viral infection, and a consequence of the viral inflammatory process.