Selected phenotypic and cloning properties of a bovine lymphoblastoid cell line, BL20

A bovine lymphoblastoid cell line, BL20, was shown to express a BoLA antigen and surface IgM, implying that it was probably of B-cell origin. At low density (< 10 5/ml), the cells failed to grow. Inclusion of growth factor-containing supernatants from concanavalin A or pokeweed mitogen (PWM)-acti...

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Published inVeterinary immunology and immunopathology Vol. 20; no. 2; pp. 165 - 172
Main Authors Olobo, J.O., Black, S.J.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 1989
Subjects
Animals
Antigens, Surface - analysis
B-Lymphocytes - cytology
B-Lymphocytes - immunology
BOVIN
CATTLE
CELL CULTURE
Cell Division
Cell Line
Cell Survival
CLONACION
CLONAGE
Clone Cells
CLONING
CULTIVO DE CELULAS
CULTURE DE CELLULES
Fibroblasts
Fluorescent Antibody Technique
GANADO BOVINO
Immunoglobulins - analysis
IMMUNOLOGIE
IMMUNOLOGY
INMUNOLOGIA
Phenotype
Thymus Gland - cytology
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Summary:A bovine lymphoblastoid cell line, BL20, was shown to express a BoLA antigen and surface IgM, implying that it was probably of B-cell origin. At low density (< 10 5/ml), the cells failed to grow. Inclusion of growth factor-containing supernatants from concanavalin A or pokeweed mitogen (PWM)-activated bovine lymphocytes or from thymus fibroblast-like cells did not improve cloning of the cells. Feeder cells also did not enhance cloning of the cells except for bovine thymus fibroblast-like cell monolayer.
Bibliography:L70
8901488
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ISSN:0165-2427
1873-2534
DOI:10.1016/0165-2427(89)90096-2