Comparison of Biochemical, Anatomical, Morphological, and Physiological Responses to Salinity Stress in Wheat and Barley Genotypes Deferring in Salinity Tolerance

• Published in: Agronomy (Basel) Vol. 10; no. 1; p. 127
• Main Authors: Zeeshan, Muhammad, Lu, Meiqin, Sehar, Shafaque, Holford, Paul, Wu, Feibo
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.01.2020
• Subjects: Anopheles; Antioxidants; Antioxidants (Nutrients); Ascorbic acid; Barley; Catalase; Chlorophyll; Cultivars; Enzymes; Experiments; Gene expression; Genetic aspects; Genotypes; Glutathione; Glutathione reductase; Hydroponics; L-Ascorbate peroxidase; Leaves; Lipid peroxidation; Lipids; Malondialdehyde; Morphology; osmotic stress; Peroxidase; Photosynthesis; Physiological responses; Physiology; Plant growth; Reductases; Reduction; Salinity; Salinity effects; Salinity tolerance; Salt; Salts; Seeds; Sodium chloride; Superoxide dismutase; Superoxides; Time; Ultrastructure; Wheat; Australia; China; United States > US
• ISSN: 2073-4395; 2073-4395
• DOI: 10.3390/agronomy10010127

A greenhouse hydroponic experiment was performed using salt-tolerant (cv. Suntop) and -sensitive (Sunmate) wheat cultivars and a salt-tolerant barley cv. CM72 to evaluate how cultivar and species differ in response to salinity stress. Results showed that wheat cv. Suntop performed high tolerance to salinity, being similar tolerance to salinity with CM72, compared with cv. Sunmate. Similar to CM72, Suntop recorded less salinity induced increase in malondialdehyde (MDA) accumulation and less reduction in plant height, net photosynthetic rate (Pn), chlorophyll content, and biomass than in sensitive wheat cv. Sunmate. Significant time-course and cultivar-dependent changes were observed in the activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) in roots and leaves after salinity treatment. Higher activities were found in CM72 and Suntop compared to Sunmate. Furthermore, a clear modification was observed in leaf and root ultrastructure after NaCl treatment with more obvious changes in the sensitive wheat cv. Sunmate, rather than in CM72 and Suntop. Although differences were observed between CM72 and Suntop in the growth and biochemical traits assessed and modified by salt stress, the differences were negligible in comparison with the general response to the salt stress of sensitive wheat cv. Sunmate. In addition, salinity stress induced an increase in the Na+ and Na+/K+ ratio but a reduction in K+ concentrations, most prominently in Sunmate and followed by Suntop and CM72.