Pb(NO3)2 induces cell apoptosis through triggering of reactive oxygen species accumulation and disruption of mitochondrial function via SIRT3/SOD2 pathways

Lead (Pb) is nonbiodegradable and toxic to the lungs. To investigate the potential mechanisms of Pb‐induced reactive oxygen species (ROS) accumulation and cell death in the lungs, human non‐small lung carcinoma H460 cells were stimulated with Pb(NO3)2 in this study. The results showed that Pb(NO3)2...

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Published inEnvironmental toxicology Vol. 39; no. 3; pp. 1294 - 1302
Main Authors Lin, Hui‐Wen, Lee, Hsiang‐Lin, Shen, Ting‐Jing, Ho, Meng‐Ting, Lee, Yi‐Ju, Wang, Inga, Lin, Ching‐Pin, Chang, Yuan‐Yen
Format Journal Article
LanguageEnglish
Published Hoboken, USA John Wiley & Sons, Inc 01.03.2024
Wiley Subscription Services, Inc
Subjects
Accumulation
Apoptosis
Caspase-3
cell apoptosis
Cell death
Cells
Disulfide reductase
Glutathione
Hydrogen peroxide
Lead
Lung carcinoma
Lungs
Membrane potential
Mitochondria
mitochondrial
Mortality
Neoplasms
oxidoreductive activity
Oxygen
Pb(NO3)2
Reactive oxygen species
Reductases
ROS
Superoxide dismutase
cell apoptosis
mitochondrial
oxidoreductive activity
Pb(NO3)2
ROS
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Summary:Lead (Pb) is nonbiodegradable and toxic to the lungs. To investigate the potential mechanisms of Pb‐induced reactive oxygen species (ROS) accumulation and cell death in the lungs, human non‐small lung carcinoma H460 cells were stimulated with Pb(NO3)2 in this study. The results showed that Pb(NO3)2 stimulation increased cell death by inducing cell apoptosis which showed a reduced Bcl‐2 expression and an enhanced caspase 3 activation. Pb(NO3)2 also caused the production of H2O2 in H460 cells that triggering the buildup of ROS and mitochondrial membrane potential loss. We found that Pb(NO3)2 modulates oxidoreductive activity through reduced the glutathione‐disulfide reductase and glutathione levels in Pb(NO3)2‐exposed H460 cells. Furthermore, the superoxide dismutase (SOD) upstream molecule sirtuin 3 (SIRT3) was increased with Pb(NO3)2 dose. Collectively, these results demonstrate that Pb(NO3)2 promotes lung cell death through SIRT3/SOD‐mediated ROS accumulation and mitochondrial dysfunction.
Bibliography:Hui‐Wen Lin and Hsiang‐Lin Lee contributed equally as first authors.
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ISSN:1520-4081
1522-7278
DOI:10.1002/tox.24019