Measurement of the rate of uptake and subcellular localization of porphyrins in cells using fluorescence digital imaging microscopy

A fluorescence imaging system incorporating a cooled slow-scan charge-coupled device camera was used to study the rate of uptake and subcellular localization of prophyrins in living cells. Measurements were carried out on human dermal fibroblasts (D532) using two different prophyrins meso-tetra(4-N-...

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Published inPhotochemistry and photobiology Vol. 59; no. 4; p. 419
Main Authors Georgiou, G N, Ahmet, M T, Houlton, A, Silver, J, Cherry, R J
Format Journal Article
LanguageEnglish
Published United States 01.04.1994
Subjects
Cells, Cultured
Humans
Image Processing, Computer-Assisted
Microscopy, Fluorescence
Porphyrins - pharmacokinetics
Subcellular Fractions - metabolism
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Summary:A fluorescence imaging system incorporating a cooled slow-scan charge-coupled device camera was used to study the rate of uptake and subcellular localization of prophyrins in living cells. Measurements were carried out on human dermal fibroblasts (D532) using two different prophyrins meso-tetra(4-N-methylpyridyl)porphine (TMPP) and meso-tetra(4-N-hexylpyridyl)porphine (THPP). It was observed that TMPP was rapidly taken up by cells and principally located in the nucleus. The THPP, on the other hand, internalized more slowly and exhibited a particulate distribution in the cytoplasm.
ISSN:0031-8655
DOI:10.1111/j.1751-1097.1994.tb05058.x