Intraoperative peritoneal washing cytology with the rapid immunoperoxidase method using microwave irradiation

The rapid immunocytochemical staining of gastrointestinal cancer-associated antigens, carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9), for intraoperative peritoneal washing cytology of pancreatic or biliary carcinoma can be completed in only 25 minutes by using microwave irradi...

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Bibliographic Details
Published inJournal of surgical oncology Vol. 60; no. 1; p. 30
Main Authors Nomoto, S, Nakao, A, Takeuchi, Y, Nonami, T, Harada, A, Ichihara, T, Takagi, H
Format Journal Article
LanguageEnglish
Published United States 01.09.1995
Subjects
Aged
Ascitic Fluid - pathology
Biliary Tract Neoplasms - pathology
Biliary Tract Neoplasms - surgery
CA-19-9 Antigen - analysis
Carcinoembryonic Antigen - analysis
Female
Humans
Immunoenzyme Techniques
Immunohistochemistry
Intraoperative Period
Male
Microwaves
Middle Aged
Pancreatic Neoplasms - pathology
Pancreatic Neoplasms - surgery
Staining and Labeling
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Summary:The rapid immunocytochemical staining of gastrointestinal cancer-associated antigens, carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9), for intraoperative peritoneal washing cytology of pancreatic or biliary carcinoma can be completed in only 25 minutes by using microwave irradiation to accelerate incubation of the primary antibody. Only 3-second irradiation at 500 W for fresh smears produced specific antigen staining of malignant cells similar to that obtained with longer incubation, and the sensitivity of intraoperative cytology was greater than by the conventional staining method. The rapid immunocytochemical staining method of peritoneal washings at laparotomy can provide sensitive information for determining more aggressive therapy for intraperitoneal spread of malignant cells before closing the abdominal wall.
ISSN:0022-4790
DOI:10.1002/jso.2930600107