Bumetanide binding to normal albumins and to albumin in renal failure

Binding of bumetanide, a loop diuretic, to partially purified albumins from renal failure patients (RF-HA), and healthy subjects (N-HA), human serum albumin (HSA) and defatted-HSA (D-HSA), was studied with equilibrium dialysis at a constant albumin concentration and various ligand concentrations. Bi...

Full description

Saved in:
Bibliographic Details
Published inBiopharmaceutics & drug disposition Vol. 14; no. 6; p. 503
Main Authors Howlett, M R, Auld, W H, Murdoch, W R, Skellern, G G
Format Journal Article
LanguageEnglish
Published England 01.08.1993
Subjects
Albumins - metabolism
Bumetanide - metabolism
Humans
Kidney Failure, Chronic - blood
Kinetics
Polyethylene Glycols
Protein Binding
Serum Albumin - isolation & purification
Serum Albumin - metabolism
Online AccessGet more information

Cover

More Information
Summary:Binding of bumetanide, a loop diuretic, to partially purified albumins from renal failure patients (RF-HA), and healthy subjects (N-HA), human serum albumin (HSA) and defatted-HSA (D-HSA), was studied with equilibrium dialysis at a constant albumin concentration and various ligand concentrations. Binding parameters (n and K) were estimated from Scatchard plots and with a non-linear two-binding site model computer program, assuming two classes of independent sites. The binding capacities (n1K1) decreased in the order N-HA > RF-HA > D-HSA > HSA. Computer estimates of K1 for the partially purified albumin preparations were not markedly different. However, the graphical estimate of K1 for N-HA was greater than that for RF-HA. When the degree of binding (r) was plotted as a function of the logarithm of the free bumetanide concentration, an asymptotic plateau was not observed, indicating that the protein binding sites were not saturated. Consequently, the calculated binding estimates may not adequately describe the binding of bumetanide.
ISSN:0142-2782
DOI:10.1002/bdd.2510140606