Quantification of Maternal Microchimeric Cells in the Liver of Children With Biliary Atresia

• Published in: Journal of pediatric gastroenterology and nutrition Vol. 74; no. 4; pp. e83 - e86
• Main Authors: Tamaoka, Satoshi, Fukuda, Akinari, Katoh‐Fukui, Yuko, Hattori, Atsushi, Uchida, Hajime, Shimizu, Seiichi, Yanagi, Yusuke, Kanaan, Sami B., Sakamoto, Seisuke, Kasahara, Mureo, Yoshioka, Takako, Fukami, Maki
• Format: Journal Article
• Language: English
• Published: United States 01.04.2022
• Subjects: Biliary Atresia - etiology; Biliary Atresia - surgery; Child; Chimerism; etiology; graft tolerance; Humans; Liver; liver transplantation; Liver Transplantation - adverse effects; microchimerism
• ISSN: 0277-2116; 1536-4801; 1536-4801
• DOI: 10.1097/MPG.0000000000003388

ABSTRACT Biliary atresia (BA) is a rare disorder of unknown etiology. There is a debate as to whether maternal microchimerism plays a significant role in the development of BA or in graft tolerance after liver transplantation. Here, we performed quantitative‐PCR‐based assays for liver tissues of children with BA and other diseases. Maternal cells were detected in 4/13 and 1/3 of the BA and control groups, respectively. The estimated number of maternal cells ranged between 0 and 34.7 per 106 total cells. The frequency and severity of maternal microchimerism were similar between the BA and control groups, and between patients with and without acute rejection of maternal grafts. These results highlight the high frequency of maternal microchimerism in the liver. This study provides no evidence for roles of microchimerism in the etiology of BA or in graft tolerance. Thus, the biological consequences of maternal microchimerism need to be clarified in future studies.