Photo-damage protective effect of two facial products, containing a unique complex of Dead Sea minerals and Himalayan actives

• Published in: Journal of cosmetic dermatology Vol. 11; no. 3; pp. 183 - 192
• Main Authors: Wineman, Eitan, Portugal-Cohen, Meital, Soroka, Yoram, Cohen, Dror, Schlippe, Gerrit, Voss, Werner, Brenner, Sarah, Milner, Yoram, Hai, Noam, Ma'or, Zeevi
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.2012
• Subjects: Administration, Cutaneous; Adult; Antioxidants - pharmacology; apoptosis; Apoptosis - drug effects; Apoptosis - radiation effects; Caspase 3 - metabolism; Cosmetics - pharmacology; Dead Sea minerals; Female; Fruit; Himalayan extracts; Humans; inflammation; Lichens; Lycium; Middle Aged; Mineral Waters; photo-damage; Plant Extracts - pharmacology; Plant Roots; reactive oxygen species (ROS); Skin Aging - drug effects; Skin Aging - pathology; Skin Aging - radiation effects; Tissue Culture Techniques; Tumor Necrosis Factor-alpha - radiation effects; Tumor Necrosis Factor-alpha - secretion; Ultraviolet Rays - adverse effects; Young Adult
• ISSN: 1473-2130; 1473-2165; 1473-2165
• DOI: 10.1111/j.1473-2165.2012.00625.x

Summary Background  Skin appearance is badly affected when exposed to solar UV rays, which encourage physiological and structural cutaneous alterations that eventually lead to skin photo‐damage. Aims  To test the capability of two facial preparations, extreme day cream (EXD) and extreme night treatment (EXN), containing a unique complex of Dead Sea water and three Himalayan extracts, to antagonize biological effects induced by photo‐damage. Methods  Pieces of organ cultures of human skin were used as a model to assess the biological effects of UVB irradiation and the protective effect of topical application of two Extreme preparations. Skin pieces were analyzed for mitochondrial activity by MTT assay, for apoptosis by caspase 3 assay, and for cytokine secretion by solid phase ELISA. Human subjects were tested to evaluate the effect of Extreme preparations on skin wrinkle depth using PRIMOS and skin hydration by a corneometer. Results  UVB irradiation induced cell apoptosis in the epidermis of skin organ cultures and increased their pro‐inflammatory cytokine, tumor necrosis α (TNFα) secretion. Topical applications of both preparations significantly attenuated all these effects. Furthermore, in human subjects, a reduction in wrinkle depth and an elevation in the intense skin moisture were observed. Conclusions  The observations clearly show that EXD and EXN preparations have protective anti‐apoptotic and anti‐inflammatory properties that can attenuate biological effects of skin photo‐damage. Topical application of the preparations improves skin appearance by reducing its wrinkles depth and increasing its moisturizing impact.