Determination of small sample volumes in the analysis of drugs at specific sites in the oral cavity

Two methods for the determination of sample volumes between 0.2 and 0.6 microliters were compared by preparing standard curves for volumes over this range. The first method used a Periotron and the second the sample mass. A linear model was fitted and 95% confidence limits for volumes estimated by e...

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Bibliographic Details
Published inJournal of periodontal research Vol. 30; no. 2; p. 144
Main Authors Medlicott, N J, Tucker, I G, Rathbone, M J, Holborow, D W
Format Journal Article
LanguageEnglish
Published United States 01.03.1995
Subjects
Delayed-Action Preparations - analysis
Humans
Least-Squares Analysis
Linear Models
Mouth
Saliva - chemistry
Specimen Handling - instrumentation
Weights and Measures
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Summary:Two methods for the determination of sample volumes between 0.2 and 0.6 microliters were compared by preparing standard curves for volumes over this range. The first method used a Periotron and the second the sample mass. A linear model was fitted and 95% confidence limits for volumes estimated by each method were calculated. This showed that use of either the maximum Periotron reading or the sample mass allowed estimation of volumes to within +/- 0.056 microliter and +/- 0.047 microliter respectively. It is proposed that measurement of sample mass provides a simple and accurate method to determine sample volume when analysing drug concentrations at specific sites in the oral cavity.
ISSN:0022-3484
DOI:10.1111/j.1600-0765.1995.tb01264.x