Extracellular and Circulating Redox- and Metalloregulated eRNA and eRNP: Copper Ion-Structured RNA Cytokines (Angiotropin Ribokines) and Bioaptamer Targets Imparting RNA Chaperone and Novel Biofunctions to S100-EF-Hand and Disease-Associated Proteins

• Published in: Annals of the New York Academy of Sciences Vol. 1022; no. 1; pp. 163 - 184
• Main Author: WISSLER, JOSEF H.
• Format: Journal Article Book Chapter
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.06.2004
• Subjects: 3D-rapid prototyping molecular image model; adenosine N-oxide; aging; Alzheimer's amyloid precursor; Amino Acid Sequence; amylotrophic lateral sclerosis; angiogenesis; angiotropin CuRNP ribokine; Base Sequence; Binding Sites; bioaptamer; calgranulin; Cells, Cultured; central dogma; Computational Biology; Copper - chemistry; Copper - metabolism; copper-ribonucleoprotein; Crystallography, X-Ray; Cu, zinc transition metalloregulation; cytokine; Cytokines - chemistry; Cytokines - metabolism; DTH delayed-type hypersensitivity; EF Hand Motifs; extracellular eRNA; Feedback, Physiological; Fenton-redox reaction; fragile X syndrome; GARS-AIRS-GART; Gene Expression Regulation; Huntington's huntingtin; Imaging, Three-Dimensional; isoguanosine; Models, Molecular; modified and edited RNA bioaptamer; Molecular Sequence Data; morphogenesis; neuroprotein; Nuclear Magnetic Resonance, Biomolecular; nucleic acid-binding K3H-R3H domain; Oxidation-Reduction; Parkinson's parkin; prion PrP; proteasome; Protein Conformation; Protein Folding; Proteomics; psoriasin; receptor for advanced glycosylation end products RAGE; Recombinant Proteins - chemistry; Recombinant Proteins - metabolism; Ribonucleoproteins - chemistry; Ribonucleoproteins - metabolism; ribosomal protein; RNA - chemistry; RNA - metabolism; RNA chaperone; S100 EF-hand protein; S100 Proteins - chemistry; S100 Proteins - metabolism; Sequence Homology, Amino Acid; transfer factor; ubiquitinylation; zinc transition metalloregulation
• ISSN: 0077-8923; 1749-6632
• DOI: 10.1196/annals.1318.027

: Bioassays for cellular differentiation and tissue morphogenesis were used to design methods for isolation of bioactive redox‐ and metalloregulated nucleic acids and copper ion complexes with proteins from extracellular, circulating, wound, and supernatant fluids of cultured cells. In extracellular biospheres, diversities of nucleic acids were found to be secreted by cells upon activation. They may reflect nucleic acid biolibraries with molecular imprints of cellular history. After removal of protein components, eRNA prototypes exuded by activated cells were sequenced. They are small, endogenous, highly modified and edited, redox‐ and metalloregulated 5′‐end phosphorylated extracellular eRNA (∼2–200 bases) with cellular, enzymic, and bioaptamer functions. Fenton‐type OH* radical redox reactions may form modified nucleotides in RNA as wobbles eRNA per se, or as copper ion‐complex with protein (e.g., S100A12‐EF‐hand protein, angiotropin‐related protein, calgranulin‐C, hippocampal neurite differentiation factor) are shown to be bioactive in vivo and in vitro as cytokines (ribokines) and as nonmitogenic angiomorphogens for endothelial cell differentiation in the formation of organoid supracellular capillary structures. As bioaptamers, copper ion‐structured eRNA imparts novel biofunctions to proteins that they do not have on their own. The origin of extracellular RNA and intermediate precursors (up to 500 bases) was traced to intracellular parent nucleic acids. Intermediate precursors with and without partial homology were found. This suggests that bioaptamers are not directly retranslatable gene products. Metalloregulated eRNA bioaptamer function was investigated by domains (e.g. 5′…CUG…3′ hairpin loop) for folding, bioactivity, and binding of protein with copper, calcium, and alkali metal ion affinity. Vice versa, metalloregulated nucleic acid‐binding domains (K3H, R3H) in proteins were identified. Interaction of protein and eRNA docking potentials were visualized by 3D‐rapid prototyping of accurate molecular image models based on crystallographic or NMR data. For S100A12‐homologous proteins, receptor‐ and metalloregulated RNA chaperone‐shaped protein assemblies were investigated. They suggest insight into signaling cascades as to how eRNA transmits its cytokine (ribokine) bioinformation from the extracellular RNA biosphere into cells. Proteomics of the extracellular RNA biosphere demonstrate the presence of nucleic acid‐binding domain homologies in defense‐, aging‐, and disease‐associated neuronal and other proteins as targets for RNA orphans. By structural relationships found to transmissible processes, proteinaceous transfer (“infectivity”) and feedback of bioinformation beyond the central dogma of molecular biology are considered in terms of metalloregulated RNA bioaptamer function, nucleic acid‐binding domains, and protein conformation.