Fructose‐1,6‐bisphosphate reverses hypotensive effect caused by L‐kynurenine in Wistar male rats

• Published in: Physiological reports Vol. 12; no. 19; pp. e70033 - n/a
• Main Authors: Catarina, Anderson Velasque, Branchini, Gisele, Caceres, Rafael Andrade, Fernandes, Renata Streck, Costa, Bruna Pasqualotto, Machado, Kleiton Lima De Godoy, Becker, Tiago, Ferreira, Luis Fernando, Rigatto, Katya, Oliveira, Jarbas Rodrigues, Nunes, Fernanda Bordignon
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.10.2024; John Wiley and Sons Inc; Wiley
• Subjects: Amino acids; Animals; Blood pressure; Blood Pressure - drug effects; Calcium (blood); Calcium channels; Cardiovascular system; Free energy; Fructose; Fructosediphosphates - metabolism; Fructosediphosphates - pharmacology; fructose‐1,6‐bisphosphate; Heart rate; Heart Rate - drug effects; Hemodynamics; Hypotension; Hypotension - drug therapy; Hypotension - metabolism; Hypotension - physiopathology; Immunomodulation; Inflammation; Ion channels; KCNQ4 protein; Kynurenine - metabolism; Kynurenine - pharmacology; Laboratory animals; L‐kynurenine; Male; Molecular Docking Simulation; Original; Physiology; Potassium; Potassium channels (voltage-gated); potassium‐voltage‐gated channels; Proteins; Rats; Rats, Wistar; Regular Manuscript; Simulation; Tumor necrosis factor-TNF; Variance analysis; Vasodilation; hypotension; fructose‐1,6‐bisphosphate; inflammation; potassium‐voltage‐gated channels; L‐kynurenine
• ISSN: 2051-817X; 2051-817X
• DOI: 10.14814/phy2.70033

Hypotension is one of the main characteristics of the systemic inflammation, basically caused by endothelial dysfunction. Studies have shown that the amino acid L‐kynurenine (KYN) causes vasodilation in mammals, leading to hypotensive shock. In hypotensive shock, when activated by the KYN, the voltage‐gated potassium channel encoded by the family KCNQ (Kv7) gene can cause vasodilation. Fructose‐1,6‐bisphosphate (FBP) it is being considered in studies an anti‐inflammatory, antioxidant, immunomodulator, and a modulator of some ion channels (Ca2+, Na+, and K+). We analyzed the effects of KYN and FBP on mean blood pressure (MBP), systolic and diastolic (DBP) blood pressure, and heart rate variability (HRV) in Wistar rats. Results demonstrated that the administration of KYN significant decreased MBP, DBP, and increased HRV. Importantly, the FBP treatment reversed the KYN effects on MBP, DBP, and HRV. Molecular Docking Simulations suggested that KYN and FBP present a very close estimated free energy of binding and the same position into structure of KCNQ4. Our results did demonstrate that FBP blunted the decrease in BP, provoked by KYN. Results raise new hypotheses for future and studies in the treatment of hypotension resulting from inflammation.