Nanog-like Regulates Endoderm Formation through the Mxtx2-Nodal Pathway

• Published in: Developmental cell Vol. 22; no. 3; pp. 625 - 638
• Main Authors: Xu, Cong, Fan, Zi Peng, Müller, Patrick, Fogley, Rachel, DiBiase, Anthony, Trompouki, Eirini, Unternaehrer, Juli, Xiong, Fengzhu, Torregroza, Ingrid, Evans, Todd, Megason, Sean G., Daley, George Q., Schier, Alexander F., Young, Richard A., Zon, Leonard I.
• Format: Journal Article
• Language: English
• Published: Cambridge, MA Elsevier Inc 13.03.2012; Cell Press
• Subjects: Amino Acid Sequence; Animals; Biological and medical sciences; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; Embryo, Nonmammalian - metabolism; Endoderm - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Developmental; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Intracellular Signaling Peptides and Proteins - metabolism; Molecular and cellular biology; Molecular Sequence Data; Nanog Homeobox Protein; Nodal Signaling Ligands - metabolism; Zebrafish - embryology; Zebrafish - genetics; Zebrafish - metabolism; Zebrafish Proteins - genetics; Zebrafish Proteins - metabolism; Development; Regulation(control); Endoderm
• ISSN: 1534-5807; 1878-1551
• DOI: 10.1016/j.devcel.2012.01.003

In mammalian embryonic stem cells, the acquisition of pluripotency is dependent on Nanog, but the in vivo analysis of Nanog has been hampered by its requirement for early mouse development. In an effort to examine the role of Nanog in vivo, we identified a zebrafish Nanog ortholog and found that its knockdown impaired endoderm formation. Genome-wide transcription analysis revealed that nanog-like morphants fail to develop the extraembryonic yolk syncytial layer (YSL), which produces Nodal, required for endoderm induction. We examined the genes that were regulated by Nanog-like and identified the homeobox gene mxtx2, which is both necessary and sufficient for YSL induction. Chromatin immunoprecipitation assays and genetic studies indicated that Nanog-like directly activates mxtx2, which, in turn, specifies the YSL lineage by directly activating YSL genes. Our study identifies a Nanog-like-Mxtx2-Nodal pathway and establishes a role for Nanog-like in regulating the formation of the extraembryonic tissue required for endoderm induction. ► Nanog-like knockdown impairs endoderm formation due to decreased Nodal signaling ► Nanog-like promotes the formation of extraembryonic tissue (YSL) that secretes Nodal ► Nanog-like's target Mxtx2 activates YSL by binding directly to YSL genes During mammalian embryogenesis, Nanog promotes early pluripotency. Xu et al. identify Nanog-like, a zebrafish Nanog ortholog, which does not affect pluripotency but is required for a Nanog-like-Mxtx2-Nodal pathway in extraembryonic tissue. This pathway is essential because it is required for proper morphogenesis and endoderm induction in the embryo.