Recording of the full-field electroretinogram in minipigs

• Published in: Veterinary ophthalmology Vol. 15; no. s2; pp. 84 - 93
• Main Authors: Augsburger, Anne-Sandrine, Haag, Valérie, Leuillet, Sébastien, Legrand, Jean-Jacques, Forster, Roy
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.2012
• Subjects: Animals; electroretinogram; Electroretinography - veterinary; Female; Light; Male; maximal combined rod-cone response; minipigs; retina; Retina - physiology; Swine; Swine, Miniature - physiology
• ISSN: 1463-5216; 1463-5224
• DOI: 10.1111/j.1463-5224.2012.01052.x

Purpose  To test a simple electroretinographic protocol on a representative sample of minipigs. Animal studied  Minipig. Procedures  Electroretinogram recordings were conducted on 162 healthy minipigs (81 males and 81 females) aged 4–6 months. After a 1.5‐h light‐adaptation period, the animals were anesthetized with general anesthesia. First, binocular full‐field photopic electroretinogram recordings were conducted under photopic conditions. Subsequently, scotopic electroretinogram recordings were conducted during dark‐adaptation periods every 4 min for a 20‐min period. At the end of this period, the maximal combined rod‐cone response was recorded by measuring the retinal response to a single high‐intensity flash. We used sclerocorneal clip electrodes as active electrodes and needle electrodes as reference and ground electrodes. Results  The a‐wave and b‐wave peak times and amplitudes have been measured and statistically analyzed. For each of the statistical comparisons, normality and homogeneity of variances were evaluated. No significant gender differences were observed, with the exception of a higher b‐wave amplitude for the photopic ERG recordings observed in females when compared to males (48.14 ± 12.909 μV vs. 42.88 ± 10.666 μV; P = 0.005). The process of dark adaptation was evaluated, and the maximal combined rod‐cone response was measured (a‐ and b‐waves amplitude and peak time). Conclusions  We conducted photopic and scotopic electroretinogram recordings from a protocol based on light adaptation followed by dark adaptation using sclerocorneal clip electrodes, which allows quick assembly and examination.