First isolation and establishment of in vitro culture of Theileria lestoquardi from a naturally infected cow

Theileria infected cell line was isolated from the prescapular lymph node of an adult crossbred cow. Molecular study confirmed this cell line of bovine lymphocyte has been transformed by the Theileria lestoquardi. This strain of T. lestoquardi designated Ka-6 and sheep were inoculated with this stra...

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Bibliographic Details
Published inJournal of parasitic diseases Vol. 39; no. 2; pp. 328 - 331
Main Authors Namavari, M., Ezhdehakosh-pour, S., Habibi, G. R., Rahimian, A., Namazi, F.
Format Journal Article
LanguageEnglish
Published New Delhi Springer India 01.06.2015
Springer Nature B.V
Subjects
Bovine spongiform encephalopathy
Cell culture
Health Promotion and Disease Prevention
Infectious Diseases
Lymph nodes
Lymphocytes
Medicine
Medicine & Public Health
Short Communication
Theileria lestoquardi
Theileriosis
Tissue culture
Cow
Cell line
Theileria lestoquardi
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Summary:Theileria infected cell line was isolated from the prescapular lymph node of an adult crossbred cow. Molecular study confirmed this cell line of bovine lymphocyte has been transformed by the Theileria lestoquardi. This strain of T. lestoquardi designated Ka-6 and sheep were inoculated with this strain didn’t show any clinical signs of theileriosis which shows the significance of this cell line to develop a tissue-culture vaccine against malignant ovine theileriosis. Contrary to accepted belief that the T. lestoquardi not capable of causing disease in cattle, the present study describes the first isolation and establishment of in vitro culture of T. lestoquardi -infected cell line from a naturally infected cow with typical singes of acute theileriosis.
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ISSN:0971-7196
0975-0703
DOI:10.1007/s12639-013-0309-2