Isolation and identification of morphine 3- and 6-glucuronides, morphine 3,6-diglucuronide, morphine 3-ethereal sulfate, normorphine, and normorphine 6-glucuronide as morphine metabolites in humans

Morphine metabolites were isolated with column chromatography on a resin and neutral aluminum oxide and TLC from the urine of morphine-dependent subjects maintained on morphine sulfate at a dose of 240 mg/day. These metabolites were characterized as morphine 3-glucuronide, morphine 6-glucuronide, mo...

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Bibliographic Details
Published inJournal of pharmaceutical sciences Vol. 66; no. 9; p. 1288
Main Authors Yeh, S Y, Gorodetzky, C W, Krebs, H A
Format Journal Article
LanguageEnglish
Published United States 01.09.1977
Subjects
Adult
Chromatography, Gas
Chromatography, Thin Layer
Glucuronates - urine
Humans
Hydrolysis
Male
Mass Spectrometry
Morphine - urine
Morphine Dependence - urine
Morphine Derivatives - urine
Phenols - urine
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Summary:Morphine metabolites were isolated with column chromatography on a resin and neutral aluminum oxide and TLC from the urine of morphine-dependent subjects maintained on morphine sulfate at a dose of 240 mg/day. These metabolites were characterized as morphine 3-glucuronide, morphine 6-glucuronide, morphine 3,6-diglucuronide, morphine 3-ethereal sulfate, normorphine, normorphine 6-glucuronide, and, possibly, normorphine 3-glucuronide by free phenol and glucuronide tests, enzymatic hydrolysis, GLC, TLC, UV spectroscopy, and GLC--mass spectrometry.
ISSN:0022-3549
DOI:10.1002/jps.2600660921