Factors interfering with the cell morphology in smears

Factors interfering with the cell morphology in smears were studied. The following artifacts were observed: Lysis of cells with release of granules, cytoplasmic and nuclear proteins; fern-like structures due to detachment of protein from glass; rupture of the surface of smears; crystal formations; l...

Full description

Saved in:
Bibliographic Details
Published inScandinavian journal of haematology Vol. 23; no. 3; p. 197
Main Authors Talstad, I, Gundersen, J S
Format Journal Article
LanguageEnglish
Published Denmark 01.09.1979
Subjects
Blood Cells - cytology
Cell Aggregation
Cell Nucleus
Crystallization
Cytoplasm
Humans
Neutrophils - cytology
Vacuoles
Online AccessGet more information

Cover

Abstract Factors interfering with the cell morphology in smears were studied. The following artifacts were observed: Lysis of cells with release of granules, cytoplasmic and nuclear proteins; fern-like structures due to detachment of protein from glass; rupture of the surface of smears; crystal formations; leakage from cells interfering with the surrounding medium; cell shrinkage, vacuolization; and aggregation of cells caused by gammaglobulins. The concentration and type of plasma protein in the medium greatly influenced the type of artifacts. The technique by which the least artifacts occurred, was cytocentrifugation of cells suspended in a cell protecting medium.
AbstractList Factors interfering with the cell morphology in smears were studied. The following artifacts were observed: Lysis of cells with release of granules, cytoplasmic and nuclear proteins; fern-like structures due to detachment of protein from glass; rupture of the surface of smears; crystal formations; leakage from cells interfering with the surrounding medium; cell shrinkage, vacuolization; and aggregation of cells caused by gammaglobulins. The concentration and type of plasma protein in the medium greatly influenced the type of artifacts. The technique by which the least artifacts occurred, was cytocentrifugation of cells suspended in a cell protecting medium.
Author Gundersen, J S
Talstad, I
Author_xml – sequence: 1
  givenname: I
  surname: Talstad
  fullname: Talstad, I
– sequence: 2
  givenname: J S
  surname: Gundersen
  fullname: Gundersen, J S
BackLink https://www.ncbi.nlm.nih.gov/pubmed/504967$$D View this record in MEDLINE/PubMed
BookMark eNotjr1OwzAYRT2Un1L6BgwWe8Jnx_4cj6iiBakSC0hsle3YTar8yQ6Cvj2RylnucnR17siiH3pPyCODnM08nXKGABkg6JxppfPJAkfN8t8FWQIUmElZfN2SdUonmEEJnKkbci1BaFRLglvjpiEm2vSTj8HHpj_Sn2aq6VR76nzb0m6IYz20w_E8SzR13sR0T66CaZNf_--KfG5fPjav2f5997Z53mdOgGKZ5I4zhlorEwqL3KK1pVMOBEcRuPfKBvCydEVAYYuqRI6mQiZAmspjxVfk4fI7ftvOV4cxNp2J58Mln_8BU7NJ9A
CitedBy_id crossref_primary_10_1111_j_1365_3083_1990_tb02821_x
crossref_primary_10_1111_j_1600_0609_1981_tb01681_x
crossref_primary_10_1016_0022_1759_81_90254_4
crossref_primary_10_1016_0022_1759_84_90385_5
ContentType Journal Article
DBID CGR
CUY
CVF
ECM
EIF
NPM
DOI 10.1111/j.1600-0609.1979.tb02691.x
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
DatabaseTitleList MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod no_fulltext_linktorsrc
Discipline Medicine
ExternalDocumentID 504967
Genre Journal Article
GroupedDBID .55
.GJ
.Y3
1OB
1OC
3O-
53G
5RE
8-1
AAQQT
ACBWZ
ACGFS
ACXQS
AFBPY
AHEFC
ALMA_UNASSIGNED_HOLDINGS
AZFZN
BDRZF
CGR
CUY
CVF
ECM
EGARE
EIF
F5P
FZ0
GODZA
J5H
LW6
NPM
PALCI
RIWAO
SAMSI
WXSBR
X7M
ZGI
ZXP
ID FETCH-LOGICAL-c4071-52c2116997af3b62b6bb8c7c04264f2ee7bf0e58c3f64b3d8626ad61405ade6d2
ISSN 0036-553X
IngestDate Thu May 23 23:53:35 EDT 2024
IsPeerReviewed true
IsScholarly true
Issue 3
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c4071-52c2116997af3b62b6bb8c7c04264f2ee7bf0e58c3f64b3d8626ad61405ade6d2
PMID 504967
ParticipantIDs pubmed_primary_504967
PublicationCentury 1900
PublicationDate September 1979
PublicationDateYYYYMMDD 1979-09-01
PublicationDate_xml – month: 09
  year: 1979
  text: September 1979
PublicationDecade 1970
PublicationPlace Denmark
PublicationPlace_xml – name: Denmark
PublicationTitle Scandinavian journal of haematology
PublicationTitleAlternate Scand J Haematol
PublicationYear 1979
SSID ssj0000650217
Score 1.2298236
Snippet Factors interfering with the cell morphology in smears were studied. The following artifacts were observed: Lysis of cells with release of granules,...
SourceID pubmed
SourceType Index Database
StartPage 197
SubjectTerms Blood Cells - cytology
Cell Aggregation
Cell Nucleus
Crystallization
Cytoplasm
Humans
Neutrophils - cytology
Vacuoles
Title Factors interfering with the cell morphology in smears
URI https://www.ncbi.nlm.nih.gov/pubmed/504967
Volume 23
hasFullText
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ1LS8NAEIAXqyK9iI-Kb3LwVlLSJLvJHkWsRWgvttBb2Sf20Ae2evDXO_swicXi4xLCbkiy-YbJzDAzi9ANS3VEpBQh0UyGKctUSEGwQq1YTjDNObXtmnp90h2mjyM8KncqtNUlK94S79_WlfyHKowBV1Ml-weyxU1hAM6BLxyBMBx_xbjjN8sxPR9etG0q6AOrAN-E5JvTOXxH12ZpMmsupyDXy6o9-iRsWQt7m_iAvDdOn5np5fol5D6A5aycQBSR1odXWxvjCzx8GFW6orqsTJIq9GJCQoztzryFXnR1wJ5_UlFybZdRu1n5ElOtTiLaMs8yseeY0LZLy6xQWUwtFgw-Csl-mltriu0maqiW5Uat9X2Ixv1-sfGzPpstm1X5ZrM-g2vD29XRrrvrmoNhDY3BAdr3HkJw63Afoi01O0J7PZ8DcYyIpx5UqAeGegDUA0M9KKnDRYGj3kDDzv3grhv63S9CYZzsEMcCnHNCacZ0wknMCee5yIRxelMdK5VxHSmci0STlCfSuKZMgrUVYSYVkfEJ2p7NZ-oUBZomqWQ0SVKhU8baLJc5FgzHijMW0fwMNdyKxwvX4mTsvsT5hvELVC-l6BLtaBBAdQXG2YpfWxgfBiw2kQ
link.rule.ids 786
linkProvider National Library of Medicine
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Factors+interfering+with+the+cell+morphology+in+smears&rft.jtitle=Scandinavian+journal+of+haematology&rft.au=Talstad%2C+I&rft.au=Gundersen%2C+J+S&rft.date=1979-09-01&rft.issn=0036-553X&rft.volume=23&rft.issue=3&rft.spage=197&rft_id=info:doi/10.1111%2Fj.1600-0609.1979.tb02691.x&rft_id=info%3Apmid%2F504967&rft_id=info%3Apmid%2F504967&rft.externalDocID=504967
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0036-553X&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0036-553X&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0036-553X&client=summon