Factors interfering with the cell morphology in smears

Factors interfering with the cell morphology in smears were studied. The following artifacts were observed: Lysis of cells with release of granules, cytoplasmic and nuclear proteins; fern-like structures due to detachment of protein from glass; rupture of the surface of smears; crystal formations; l...

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Bibliographic Details
Published inScandinavian journal of haematology Vol. 23; no. 3; p. 197
Main Authors Talstad, I, Gundersen, J S
Format Journal Article
LanguageEnglish
Published Denmark 01.09.1979
Subjects
Blood Cells - cytology
Cell Aggregation
Cell Nucleus
Crystallization
Cytoplasm
Humans
Neutrophils - cytology
Vacuoles
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Summary:Factors interfering with the cell morphology in smears were studied. The following artifacts were observed: Lysis of cells with release of granules, cytoplasmic and nuclear proteins; fern-like structures due to detachment of protein from glass; rupture of the surface of smears; crystal formations; leakage from cells interfering with the surrounding medium; cell shrinkage, vacuolization; and aggregation of cells caused by gammaglobulins. The concentration and type of plasma protein in the medium greatly influenced the type of artifacts. The technique by which the least artifacts occurred, was cytocentrifugation of cells suspended in a cell protecting medium.
ISSN:0036-553X
DOI:10.1111/j.1600-0609.1979.tb02691.x