Characterization of acetylcholinesterase from Arthrobacter ilicis associated with the marine sponge (Spirastrella sp.)

• Published in: Journal of applied microbiology Vol. 84; no. 3; pp. 393 - 398
• Main Authors: Mohapatra, B.R., Bapuji, M.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.03.1998; Blackwell Science
• Subjects: Arthrobacter ilicis; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Fundamental and applied biological sciences. Psychology; Marine; Miscellaneous; Mission oriented research; Spirastrella; ISW, India, Andaman Is., Ritchie's Archipelago, Havelock I; ISW, Indian Ocean; Temperature; Enzyme; Environmental factor; Esterases; Acetylcholinesterase; Extracellular; Carboxylic ester hydrolases; Marine environment; Enzymatic activity; Porifera; Production; pH; Bacteria; Hydrolases; Isolate; Actinomycetes; Cations; Organophosphorus compounds; Inhibition; Invertebrata; Carbamate
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1046/j.1365-2672.1998.00360.x

The bacterium Arthrobacter ilicis isolated from the marine sponge Spirastrella sp. produces extracellular serine type acetylcholinesterase. The maximum enzyme activity was found at 45 °C and pH 8·0. The activation and deactivation energies, calculated from an Arrhenius plot, were 13·68 and 36·96 kcal mol−1, respectively. The enzyme was not affected by the addition of the major cations of sea water, such as Ca2+ and Mg2+ at 25 mmol l−1, and was strongly inhibited by EDTA and different organophosphorus and carbamate compounds at 5 mmol l−1.