Characterization of acetylcholinesterase from Arthrobacter ilicis associated with the marine sponge (Spirastrella sp.)
The bacterium Arthrobacter ilicis isolated from the marine sponge Spirastrella sp. produces extracellular serine type acetylcholinesterase. The maximum enzyme activity was found at 45 °C and pH 8·0. The activation and deactivation energies, calculated from an Arrhenius plot, were 13·68 and 36·96 kca...
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Published in | Journal of applied microbiology Vol. 84; no. 3; pp. 393 - 398 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Science Ltd
01.03.1998
Blackwell Science |
Subjects | |
Online Access | Get full text |
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Summary: | The bacterium Arthrobacter ilicis isolated from the marine sponge Spirastrella sp. produces extracellular serine type acetylcholinesterase. The maximum enzyme activity was found at 45 °C and pH 8·0. The activation and deactivation energies, calculated from an Arrhenius plot, were 13·68 and 36·96 kcal mol−1, respectively. The enzyme was not affected by the addition of the major cations of sea water, such as Ca2+ and Mg2+ at 25 mmol l−1, and was strongly inhibited by EDTA and different organophosphorus and carbamate compounds at 5 mmol l−1. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1364-5072 1365-2672 |
DOI: | 10.1046/j.1365-2672.1998.00360.x |