Inhibitory Effect of Methyl Methanethiosulfinate on β-Glucuronidase Activity

• Published in: Bioscience, biotechnology, and biochemistry Vol. 77; no. 11; pp. 2325 - 2327
• Main Authors: WATANABE, Yuko, MUROI, Ryoko, TSUCHIYA, Haruna, UDA, Yasushi, HASHIMOTO, Kei
• Format: Journal Article
• Language: English
• Published: England Japan Society for Bioscience, Biotechnology, and Agrochemistry 2013
• Subjects: Allium; Allium tuberosum; Allyl Compounds - chemistry; Bacterial Proteins - antagonists & inhibitors; Bacterial Proteins - chemistry; Chinese chive; Chive - chemistry; Disulfides - chemistry; Enzyme Assays; Glucuronidase - antagonists & inhibitors; Glucuronidase - chemistry; Kinetics; methyl methanethiosulfinate; Plant Leaves - chemistry; Solutions; Sulfinic Acids - chemistry; Sulfinic Acids - isolation & purification; β-glucuronidase
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.130510

Minced or cut-up leaves of Chinese chive (Allium tuberosum Rottler) contain thiosulfinates and their disproportionate reaction products. Among these organosulfur compounds, methyl methanethiosulfinate was found to be an uncompetitive inhibitor of β-glucuronidase. Approximately 80% of the enzyme activity was inhibited by methyl methanethiosulfinate at 50 µM, the IC 50 value being comparable to 3.6 µM.