Downregulation of LncRNAH19 and MiR-675 Promotes Migration and Invasion of Human Hepatocellular Carcinoma Cells through AKT/GSK-3β/Cdc25A Signaling Pathway

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 34; no. 3; pp. 363 - 369
• Main Author: 吕军 马宁 陈锡林 黄晓卉 汪谦
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.06.2014; Department of Hepatobiliary Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China; Department of Hepatobiliary Surgery, the Affiliated Hospital of Guilin Medical College, Guilin 541001, China%Department of Hepatobiliary Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China%Department of Surgical Laboratory, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
• Subjects: Blotting, Western; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; cdc25 Phosphatases - metabolism; Cell Line; Cell Line, Tumor; Cell Movement - genetics; Down-Regulation; Gene Expression Regulation, Neoplastic; Gene Knockdown Techniques; Glycogen Synthase Kinase 3 - metabolism; Glycogen Synthase Kinase 3 beta; Hep G2 Cells; HepG2细胞; Humans; Liver Neoplasms - genetics; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Medicine; Medicine & Public Health; MicroRNAs - genetics; Neoplasm Invasiveness; Proto-Oncogene Proteins c-akt - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA Interference; RNA, Long Noncoding - genetics; Signal Transduction; Western印迹; 侵袭; 信号通路; 定量RT-PCR; 聚合酶链反应; 肝癌细胞; 迁移; migration; miR-675; invasion; LncRNAH19; hepatocellular carcinoma; AKT/GSK-3β/Cdc25A signaling pathway; AKT/G SK-3β/Cdc25A signaling pathway
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-014-1284-2

Summary： LncRNAH19 has been implicated as having both oncogenic and tumor suppression properties in cancer. LncRNAH19 transcripts also serve as a precursor for miR-675. However, it is unknown whether LncRNAH19 and miR-675 are involved in the migration and invasion of hepatocellular carcinoma （HCC） cells. The purpose of this study was to investigate the effect and mechanism of LncRNAH19 and miR-675 on migration and invasion of HCC cells. The migration and invasion of HCC cells were measured by Transwell migration and invasion assays after transfection of HCC cells with miR-675 inhibitors and LncRNAH19siRNA. The levels of LncRNAH19 and miR-675 were detected by quantitative reverse transcriptase real-time polymerase chain reaction （qRT-PCR）, and the protein expression of AKT, GSK-3[3 and Cdc25A by Western blotting analysis. The expression levels of LncRNAHI9 and miR-675 were higher in MHCC-97H cells than in L02, Huh-7 and HepG2 cells. Transwell migration assay revealed that the miR-675 inhibitor and LncRNAH19siRNA could significantly increase the migration of HCC cells （P〈0.01） as compared with the control group. Transwell invasion assay demonstrated that the miR-675 inhibitor and LncRNAH19siRNA could significantly increase the invasion of HCC cells （P〈0.01） as compared with the control group. Western blotting analy- sis showed that the expression levels of AKT and Cdc25A were significantly increased （P〈0.05）, and the expression level of GSK-313 was significantly decreased （P〈0.05） after treatment with miR-675 inhibitors and LncRNAH19siRNA as compared with the control group. These findings suggested that inhibition of LncRNAH 19 and miR-675 expression can promote migration and invasion of HCC cells via AKT/GSK-3[3/Cdc25A signaling pathway.