Aqueous two-phase partitioning of xylanase produced by solid-state cultivation of Polyporus squamosus

The partitioning of xylanase, produced by solid-state cultivation of Polyporus squamosus, in aqueous two-phase system polyethylene glycol/ammonium sulphate was studied. In system 10% (w/w) polyethylene glycol 1500/20% (w/w) ammonium sulphate/70% (w/w) crude enzyme at pH 5.1 favourable partition coef...

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Published inProcess biochemistry (1991) Vol. 41; no. 1; pp. 232 - 235
Main Authors Antov, Mirjana G., Peričin, Draginja M., Dašić, Milica G.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 2006
Subjects
Aqueous two-phase system
Downstream processing
Partitioning
Polyporus squamosus
Solid-state cultivation
Xylanase
Solid-state cultivation
Partitioning
Xylanase
Aqueous two-phase system
Polyporus squamosus
Downstream processing
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Summary:The partitioning of xylanase, produced by solid-state cultivation of Polyporus squamosus, in aqueous two-phase system polyethylene glycol/ammonium sulphate was studied. In system 10% (w/w) polyethylene glycol 1500/20% (w/w) ammonium sulphate/70% (w/w) crude enzyme at pH 5.1 favourable partition coefficient and the top phase yield were obtained, 85.6% and 97.37%, respectively, followed by purification factor 4.8. This result suggests the possible integration of cultivation and primary downstream processing step by partitioning of xylanase in aqueous two-phase system.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2005.06.010