Gap Junctions Assemble in the Presence of Cytoskeletal Inhibitors, but Enhanced Assembly Requires Microtubules

• Published in: Experimental cell research Vol. 275; no. 1; pp. 67 - 80
• Main Authors: Johnson, Ross G., Meyer, Rita A., Li, Xin-Ren, Preus, Doris M., Tan, Lana, Grunenwald, Haiying, Paulson, Alicia F., Laird, Dale W., Sheridan, Judson D.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.04.2002
• Subjects: actin filament; Actins - drug effects; Actins - physiology; Actins - ultrastructure; Animals; Cell Aggregation - drug effects; cell communication; Cell Line; Colchicine - pharmacology; Connexin 43 - physiology; connexin43; Cytochalasin B - pharmacology; Cytoskeleton - drug effects; Cytoskeleton - physiology; Cytoskeleton - ultrastructure; Dogs; dye injection; Freeze Fracturing; freeze-fracture; gap junction; Gap Junctions - drug effects; Gap Junctions - physiology; Gap Junctions - ultrastructure; Green Fluorescent Proteins; Liver Neoplasms, Experimental; Luminescent Proteins; microtubule; Microtubules - drug effects; Microtubules - physiology; Microtubules - ultrastructure; Nocodazole - pharmacology; Rats; Tumor Cells, Cultured; microtubule; connexin43; cell communication; actin filament; gap junction; freeze-fracture; dye injection
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1006/excr.2002.5480

The role of cytoskeletal elements in gap junction (GJ) assembly has been studied using Novikoff hepatoma cells treated with cytochalasin B (CB) to disrupt actin filaments or with colchicine or nocodazole to disrupt microtubules. After 60 min of cell reaggregation, freeze-fracture was used to evaluate quantitatively the “initiation,” “maturation,” and “growth” phases of GJ assembly. The development of junctional permeability to fluorescent dyes was also analyzed. The only effects of CB on the structure or permeability of the developing junctions involved an elongation of GJ aggregates and a small decrease in formation plaque areas. Colchicine (but not the inactive form, lumicolchicine) prevented the enhancement of GJ growth by cholesterol, but its effect on basal growth was equivocal. Nocodazole inhibited the growth of GJ, even under basal conditions, without an effect on initiation. Nocodazole also blocked the forskolin-enhanced increase in the growth of GJs and, in living MDCK cells, reduced the movement of transport intermediates containing green fluorescent protein-tagged connexin43. Thus, neither actin filaments nor microtubules appear to restrict GJ assembly by anchoring intramembrane GJ proteins, nor are they absolutely required for functional GJs to form. However, microtubules are necessary for enhanced GJ growth and likely for facilitating connexin trafficking under basal conditions.