Phytase production by the thermophilic fungus Rhizomucor pusillus

A thermophilic fungus, Rhizomucor pusillus, isolated from composting soil, was studied for phytase production using solid-state fermentation. The optimization of phytase production was carried out by Box-Behnken design of experiments, using three independent variables (pH of medium, culture age and...

Full description

Saved in:
Bibliographic Details
Published inWorld journal of microbiology & biotechnology Vol. 20; no. 1; pp. 105 - 109
Main Authors CHADHA, B. S, HARMEET, Gulati, MANDEEP, Minhas, SAINI, H. S, SINGH, N
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.02.2004
Springer Nature B.V
Subjects
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Design of experiments
Fermentation
Fundamental and applied biological sciences. Psychology
Miscellaneous
Mission oriented research
Rhizomucor pusillus
Enzyme
3-Phytase
6-Phytase
SSF
Phosphoric monoester hydrolases
Thermophily
Esterases
Rhizomucor pusillus
Characterization
Fungi
Production
phytase production
Hydrolases
Box-Behnken design
Thallophyta
Online AccessGet full text

Cover

More Information
Summary:A thermophilic fungus, Rhizomucor pusillus, isolated from composting soil, was studied for phytase production using solid-state fermentation. The optimization of phytase production was carried out by Box-Behnken design of experiments, using three independent variables (pH of medium, culture age and incubation period), resulting in a maximal level of phytase (9.18 units/g substrate). The partially purified phytase was optimally active at 70 °C and pH 5.4, though the enzyme showed 80% activity over a wide pH range, 3.0-8.0. The phytase was found to have broad substrate specificity.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0959-3993
1573-0972
DOI:10.1023/B:WIBI.0000013319.13348.0a