Larkinella roseus sp. nov., a species of the family Cytophagaceae isolated from beach soil

• Published in: The journal of microbiology Vol. 56; no. 1; pp. 30 - 35
• Main Authors: Lee, Jae-Bong, Hong, Sumin, Lee, Seung-Yeol, Park, Su-Jin, Park, Kyeung Il, Choi, Seok-Gwan, Kim, Myung Kyum, Ten, Leonid N., Jung, Hee-Young
• Format: Journal Article
• Language: English
• Published: Seoul The Microbiological Society of Korea 2018; Springer Nature B.V; 한국미생물학회
• Subjects: Agar; Bacteria; Base Composition; Biomedical and Life Sciences; Cytophagaceae; Cytophagaceae - classification; Cytophagaceae - genetics; Cytophagaceae - isolation & purification; Cytophagaceae - metabolism; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty acids; Fatty Acids - chemistry; Fatty Acids - metabolism; Life Sciences; Lipids; Menaquinones; Microbial Systematics and Evolutionary Microbiology; Microbiological strains; Microbiology; New species; Nucleotide sequence; pH effects; Phosphatidylethanolamine; Phosphatidylethanolamines - metabolism; Phosphatidylserine; Phylogeny; Quinones; RNA, Ribosomal, 16S - genetics; rRNA 16S; Soil; Soil Microbiology; Species; 생물학; soil bacteria; Bacteroidetes; Larkinella
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-018-7476-x

The taxonomic position of bacterial strain, designated 15J16-1T3A T , recovered from a soil sample was established using a polyphasic approach. Phylogenic analysis based on the 16S rRNA gene sequence showed that strain 15J16-1T3A T belonged to the family Cytophagaceae , phylum Bacteroidetes , and was most closely related to ‘ Larkinella harenae ’ 15J9-9 (95.9% similarity), Larkinella ripae 15J11-11 T (95.6%), Larkinella bovis M2TB15 T (94.7%), Larkinella arboricola Z0532 T (93.9%), and Larkinella insperata LMG 22510 T (93.5%). Cells were rod-shaped, Gram-stain-negative, aerobic, and nonmotile. The isolate grew on NA, R2A, TSA, but not on LB agar. The strain was able to grow at temperature range from 10°C to 30°C with an optimum at 25°C and pH 6–8. Menaquinone MK-7 was the predominant respiratory quinone. The major cellular fatty acids comprised C 16:1 ω5c (48.6%) and C 15:0 iso (24.1%). Phosphatidylethanolamine, phosphatidylserine, and an unidentified lipid were the major polar lipids. The G + C content of the genomic DNA was 49.5 mol%. Strain 15J16-1T3A T could be distinguished from its closest phylogenetic neighbors based on its phenotypic, genotypic, and chemotaxonomic features. Therefore, the isolate is considered to represent a novel species in the genus Larkinella , for which the name Larkinella roseus sp. nov. is proposed. The type strain is 15J16-1T3A T (= KCTC 52004 T = JCM 31991 T ).