Monoclonal antibodies identify conserved epitopes on the polyhedrin of Heliothis zea nuclear polyhedrosis virus

• Published in: Virology (New York, N.Y.) Vol. 143; no. 2; pp. 380 - 391
• Main Authors: Huang, Yuan-Shen, Hu, Ping C., Kawanishi, C.Y.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.06.1985; Elsevier
• Subjects: ANTIBODIES; ANTICORPS; ANTICUERPOS; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; HELIOTHIS; HELIOTHIS ZEA; Microbiology; MONOCLONAL ANTIBODIES; Morphology, structure, chemical composition, physicochemical properties; POLYHEDROSIS VIRUSES; Virology; Virus; Heliothis zea; Entomopathogen; Baculoviridae; Antigenic determinant; Arthropoda; Insecta; Lepidoptera; Monoclonal antibody; Baculovirus; Invertebrata; Nuclear polyhedrosis virus
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/0042-6822(85)90378-2

Monoclonal antibodies were formed against polyhedrin from the Heliothis zea S nuclear polyhedrosis virus. The cross-reactivity of these antibodies with polyhedrins and granulins of other occluded baculoviruses indicates that group and subgroup epitopes exist. Evidence is presented that multiple copies of one epitope or a series of closely related epitopes exist on the polyhedrin molecule. The monoclonal antibodies, with the exception of one IgM-producing hybridoma clone, were all of the IgG1 isotype. Clones producing antibodies with identical patterns of reactivity with heterologous polyhedrins and/or granulins but exhibiting different affinities for the homologous antigen (HzSNPV polyhedrin) are demonstrated.