Effect of Acupuncture on CXCL8 Receptors in Rats Suffering from Embryo Implantation Failure

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 34; no. 1; pp. 91 - 98
• Main Author: 高伟娜 杨薇 刘亚飞 唐潇 呙月 王丽君 张明敏 黄光英
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2014; Institute of Integrated Traditional Chinese Medicine and Western Medicine, Huazhong University of Science and Technology, Wuhan 430030, China%Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
• Subjects: Acupuncture Therapy - methods; Animals; Blotting, Western; Embryo Implantation - drug effects; Embryo Implantation - genetics; Endometrium - drug effects; Endometrium - metabolism; Female; Gene Expression Regulation, Developmental; Hormone Antagonists - pharmacology; Immunohistochemistry; Medicine; Medicine & Public Health; Mifepristone - pharmacology; mRNA表达; Pregnancy; Progestins - pharmacology; Random Allocation; Rats; Rats, Wistar; Receptors, Interleukin-8A - genetics; Receptors, Interleukin-8A - metabolism; Receptors, Interleukin-8B - genetics; Receptors, Interleukin-8B - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Time Factors; Treatment Outcome; Up-Regulation - drug effects; 受体; 大鼠; 子宫内膜; 胚胎植入; 胚胎着床; 蛋白质表达; 针刺治疗; endometrium; acupuncture; CXCR1; embryo implantation failure; CXCR2
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-014-1237-9

To observe the effect of acupuncture on CXCL8 receptors（CXCR1 and CXCR2） in rat endometrium experiencing embryo implantation failure, 72 pregnant rats were randomly divided into four groups： normal group（N）, embryo implantation failure group（M）, acupuncture treatment group（A）, and progestin treatment group（W）. Then the rats in each group were equally randomized into a day-6（D6） group, a day-8（D8） group, and a day-10（D10） group. The rats in group M, group A, and group W were treated with mifepristone-sesame oil solution on day 1, while the rats in group N were injected with the same amount of sesame oil. Meanwhile, ＂Housanli＂ and ＂Sanyinjiao＂ were selected for acupuncture. From day 1 to the time of death, the rats in group A were fastened up and then acupuncture was administered while the rats in group N and group M were only fixed, and the rats in group W were given progestin. The number of implanted embryos was calculated. The expression of CXCR1 and CXCR2 in rat endometrium was detected by immunohistochemistry, Western blotting and real-time PCR. Compared to group N, the average number of implanted embryos, the protein and mRNA expression of CXCR1（D6, D8 and D10）, and the protein and mRNA expression of CXCR2（D8 and D10） in rat endometrium were significantly decreased in group M. Compared to group M, there was significant elevation in the average number of implanted embryos, the protein expression（D6, D8 and D10） and mRNA expression（D8） of CXCR1 in rat endometrium of group A, and the protein expression（D8 and D10） and mRNA expression（D8） of CXCR2 in rat endometrium of group W. These findings indicated that acupuncture can increase the number of implanted embryos in rats of embryo implantation failure, which may be relevant with up-regulation the expression of CXCR1 and CXCR2 at maternal-fetal interface of rats with embryo implantation failure.