Stearoyl-CoA Desaturase 1 Coding Sequences and Antisense RNA Affect Lipid Secretion in Transfected Chicken LMH Hepatoma Cells

• Published in: Archives of biochemistry and biophysics Vol. 380; no. 2; pp. 243 - 250
• Main Authors: Diot, Christian, Lefèvre, Pascal, Hervé, Christophe, Belloir, Bénédicte, Narce, Michel, Damon, Marie, Poisson, Jean-Pierre, Mallard, Jacques, Douaire, Madeleine
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.08.2000
• Subjects: Animals; antisense RNA; Base Sequence; Chickens; DNA Primers - genetics; Gene Expression; Isoenzymes - genetics; Isoenzymes - metabolism; Lipid Metabolism; Lipids - secretion; Liver - drug effects; Liver - enzymology; Liver - secretion; Liver Neoplasms, Experimental - enzymology; Liver Neoplasms, Experimental - genetics; Liver Neoplasms, Experimental - secretion; LMH cells; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; RNA, Antisense - genetics; RNA, Antisense - pharmacology; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNA, Neoplasm - genetics; RNA, Neoplasm - metabolism; stearoyl-CoA desaturase; Stearoyl-CoA Desaturase - genetics; Stearoyl-CoA Desaturase - metabolism; Transfection; triacylglycerol secretion; Tumor Cells, Cultured; antisense RNA; LMH cells; triacylglycerol secretion; stearoyl-CoA desaturase; lipid metabolism
• ISSN: 0003-9861; 1096-0384
• DOI: 10.1006/abbi.2000.1945

Hepatic stearoyl CoA desaturase (SCD) activity in chickens from a fat line is higher than that of chickens from a lean line and correlates with plasma triacylglycerol concentrations. Furthermore, in these lines, the hepatic SCD1 mRNA level is positively correlated with the adipose tissue weight. To analyze the contribution of the SCD1 gene in the regulation of adiposity in the early stages of triacylglycerol secretion, SCD1 coding sequence and antisense RNA expression vectors were transfected in LMH cells. After selection, these cells were analyzed with regard to SCD1 expression and lipid secretion. The amounts of secreted triacylglycerols and phospholipids were shown to be higher in LMH cells transfected with the SCD1 gene, but reduced in those transfected with the SCD1 antisense sequences when compared to cells transfected with the vector alone (without SCD1 sequences). These results provide direct evidence that the expression of the SCD1 gene plays a major role in the triacylglycerol and phospholipid secretion process.