Cytoplasmic poly (A) binding protein (PABPC2) critically regulates epidermal maintenance and turnover in planarian Schmidtea mediterranea

Identifying key cellular events that facilitate stem cell function and tissue organization is critical for understanding the process of regeneration. Planarians are powerful model system to study regeneration and stem cell (neoblast) function. Here, using planaria, we show that the initial events of...

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Published inDevelopment (Cambridge) Vol. 144; no. 17; pp. 3066 - 3079
Main Authors Bansal, Dhiru, Kulkarni, Jahnavi, Nadahalli, Kavana, Lakshmanan, Vairavan, Krishna, Srikar, Sasidharan, Vidyanand, Geo, Jini, Dilipkumar, Shilpa, Pasricha, Renu, Gulyani, Akash, Raghavan, Srikala, Palakodeti, Dasaradhi
Format Journal Article
LanguageEnglish
Published England The Company of Biologists Ltd 01.09.2017
Subjects
Animals
Cell Lineage
Cell Proliferation
Cytoplasm - metabolism
Epidermis
Epidermis - metabolism
Epithelium - metabolism
Extracellular matrix
Extracellular Matrix - metabolism
Gene Knockdown Techniques
Homeostasis
Models, Biological
Planarians - genetics
Planarians - metabolism
Poly(A)-Binding Protein I - metabolism
Regeneration
RNA, Messenger - genetics
RNA, Messenger - metabolism
Stem cells
Stem Cells and Regeneration
Wound Healing
Poly (A)-binding proteins
Regeneration
Epidermis
Neoblast
Planaria
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Abstract Identifying key cellular events that facilitate stem cell function and tissue organization is critical for understanding the process of regeneration. Planarians are powerful model system to study regeneration and stem cell (neoblast) function. Here, using planaria, we show that the initial events of regeneration, such as epithelialization and epidermal organization are critically regulated by a novel cytoplasmic Poly A binding protein, SMED-PABPC2. Knockdown (KD) of Smed-pabpc2 leads to defects in epidermal lineage specification, disorganization of epidermis and ECM, and deregulated wound healing resulting in the selective failure of neoblast proliferation near the wound region. Polysome profiling suggested epidermal lineage transcripts, including zfp-1, to be translationally regulated by SMED-PABPC2. Together, our results uncover a novel role of SMED-PABPC2 in the maintenance of epidermal and ECM integrity, critical for wound healing, and subsequent processes for regeneration.
AbstractList Identifying key cellular events that facilitate stem cell function and tissue organization is critical for understanding the process of regeneration. Planarians are powerful model system to study regeneration and stem cell (neoblast) function. Here, using planaria, we show that the initial events of regeneration, such as epithelialization and epidermal organization are critically regulated by a novel cytoplasmic Poly A binding protein, SMED-PABPC2. Knockdown (KD) of Smed-pabpc2 leads to defects in epidermal lineage specification, disorganization of epidermis and ECM, and deregulated wound healing resulting in the selective failure of neoblast proliferation near the wound region. Polysome profiling suggested epidermal lineage transcripts, including zfp-1, to be translationally regulated by SMED-PABPC2. Together, our results uncover a novel role of SMED-PABPC2 in the maintenance of epidermal and ECM integrity, critical for wound healing, and subsequent processes for regeneration.
Identifying key cellular events that facilitate stem cell function and tissue organization is crucial for understanding the process of regeneration. Planarians are powerful model system to study regeneration and stem cell (neoblast) function. Here, using planaria, we show that the initial events of regeneration, such as epithelialization and epidermal organization are critically regulated by a novel cytoplasmic poly A-binding protein, SMED-PABPC2. Knockdown of smed-pabpc2 leads to defects in epidermal lineage specification, disorganization of epidermis and ECM, and deregulated wound healing, resulting in the selective failure of neoblast proliferation near the wound region. Polysome profiling suggests that epidermal lineage transcripts, including zfp-1, are translationally regulated by SMED-PABPC2. Together, our results uncover a novel role for SMED-PABPC2 in the maintenance of epidermal and ECM integrity, critical for wound healing and subsequent processes for regeneration.Summary: PABPC2 plays a crucial role in the maintenance of epidermal and extracellular matrix integrity, which is essential for neoblast function and regeneration in planarians.
Identifying key cellular events that facilitate stem cell function and tissue organization is crucial for understanding the process of regeneration. Planarians are powerful model system to study regeneration and stem cell (neoblast) function. Here, using planaria, we show that the initial events of regeneration, such as epithelialization and epidermal organization are critically regulated by a novel cytoplasmic poly A-binding protein, SMED-PABPC2. Knockdown leads to defects in epidermal lineage specification, disorganization of epidermis and ECM, and deregulated wound healing, resulting in the selective failure of neoblast proliferation near the wound region. Polysome profiling suggests that epidermal lineage transcripts, including , are translationally regulated by SMED-PABPC2 Together, our results uncover a novel role for SMED-PABPC2 in the maintenance of epidermal and ECM integrity, critical for wound healing and subsequent processes for regeneration.
Identifying key cellular events that facilitate stem cell function and tissue organization is crucial for understanding the process of regeneration. Planarians are powerful model system to study regeneration and stem cell (neoblast) function. Here, using planaria, we show that the initial events of regeneration, such as epithelialization and epidermal organization are critically regulated by a novel cytoplasmic poly A-binding protein, SMED-PABPC2. Knockdown of smed-pabpc2 leads to defects in epidermal lineage specification, disorganization of epidermis and ECM, and deregulated wound healing, resulting in the selective failure of neoblast proliferation near the wound region. Polysome profiling suggests that epidermal lineage transcripts, including zfp-1 , are translationally regulated by SMED-PABPC2 . Together, our results uncover a novel role for SMED-PABPC2 in the maintenance of epidermal and ECM integrity, critical for wound healing and subsequent processes for regeneration. Summary: PABPC2 plays a crucial role in the maintenance of epidermal and extracellular matrix integrity, which is essential for neoblast function and regeneration in planarians.
Identifying key cellular events that facilitate stem cell function and tissue organization is crucial for understanding the process of regeneration. Planarians are powerful model system to study regeneration and stem cell (neoblast) function. Here, using planaria, we show that the initial events of regeneration, such as epithelialization and epidermal organization are critically regulated by a novel cytoplasmic poly A-binding protein, SMED-PABPC2. Knockdown of smed-pabpc2 leads to defects in epidermal lineage specification, disorganization of epidermis and ECM, and deregulated wound healing, resulting in the selective failure of neoblast proliferation near the wound region. Polysome profiling suggests that epidermal lineage transcripts, including zfp-1, are translationally regulated by SMED-PABPC2. Together, our results uncover a novel role for SMED-PABPC2 in the maintenance of epidermal and ECM integrity, critical for wound healing and subsequent processes for regeneration.Identifying key cellular events that facilitate stem cell function and tissue organization is crucial for understanding the process of regeneration. Planarians are powerful model system to study regeneration and stem cell (neoblast) function. Here, using planaria, we show that the initial events of regeneration, such as epithelialization and epidermal organization are critically regulated by a novel cytoplasmic poly A-binding protein, SMED-PABPC2. Knockdown of smed-pabpc2 leads to defects in epidermal lineage specification, disorganization of epidermis and ECM, and deregulated wound healing, resulting in the selective failure of neoblast proliferation near the wound region. Polysome profiling suggests that epidermal lineage transcripts, including zfp-1, are translationally regulated by SMED-PABPC2. Together, our results uncover a novel role for SMED-PABPC2 in the maintenance of epidermal and ECM integrity, critical for wound healing and subsequent processes for regeneration.
Author Krishna, Srikar
Raghavan, Srikala
Pasricha, Renu
Nadahalli, Kavana
Dilipkumar, Shilpa
Bansal, Dhiru
Sasidharan, Vidyanand
Lakshmanan, Vairavan
Palakodeti, Dasaradhi
Kulkarni, Jahnavi
Gulyani, Akash
Geo, Jini
AuthorAffiliation 4 Sastra University , Thanjavur 613402 India
5 National Centre for Biological Sciences , GKVK PO, Bellary Road, Bangalore 560065 , India
1 Institute for Stem Cell Biology and Regenerative Medicine , GKVK PO, Bellary Road, Bangalore 560065 , India
3 Transdisciplinary University , Bangalore 560064 , India
2 Manipal University , Manipal 576104 , India
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Issue 17
Keywords Poly (A)-binding proteins
Regeneration
Epidermis
Neoblast
Planaria
Language English
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2017. Published by The Company of Biologists Ltd.
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Snippet Identifying key cellular events that facilitate stem cell function and tissue organization is critical for understanding the process of regeneration....
Identifying key cellular events that facilitate stem cell function and tissue organization is crucial for understanding the process of regeneration. Planarians...
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SubjectTerms Animals
Cell Lineage
Cell Proliferation
Cytoplasm - metabolism
Epidermis
Epidermis - metabolism
Epithelium - metabolism
Extracellular matrix
Extracellular Matrix - metabolism
Gene Knockdown Techniques
Homeostasis
Models, Biological
Planarians - genetics
Planarians - metabolism
Poly(A)-Binding Protein I - metabolism
Regeneration
RNA, Messenger - genetics
RNA, Messenger - metabolism
Stem cells
Stem Cells and Regeneration
Wound Healing
Title Cytoplasmic poly (A) binding protein (PABPC2) critically regulates epidermal maintenance and turnover in planarian Schmidtea mediterranea
URI https://www.ncbi.nlm.nih.gov/pubmed/28807897
https://www.proquest.com/docview/1983414356
https://www.proquest.com/docview/1929105656
https://pubmed.ncbi.nlm.nih.gov/PMC5611960
Volume 144
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