Cytoplasmic poly (A) binding protein (PABPC2) critically regulates epidermal maintenance and turnover in planarian Schmidtea mediterranea

Identifying key cellular events that facilitate stem cell function and tissue organization is critical for understanding the process of regeneration. Planarians are powerful model system to study regeneration and stem cell (neoblast) function. Here, using planaria, we show that the initial events of...

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Published inDevelopment (Cambridge) Vol. 144; no. 17; pp. 3066 - 3079
Main Authors Bansal, Dhiru, Kulkarni, Jahnavi, Nadahalli, Kavana, Lakshmanan, Vairavan, Krishna, Srikar, Sasidharan, Vidyanand, Geo, Jini, Dilipkumar, Shilpa, Pasricha, Renu, Gulyani, Akash, Raghavan, Srikala, Palakodeti, Dasaradhi
Format Journal Article
LanguageEnglish
Published England The Company of Biologists Ltd 01.09.2017
Subjects
Animals
Cell Lineage
Cell Proliferation
Cytoplasm - metabolism
Epidermis
Epidermis - metabolism
Epithelium - metabolism
Extracellular matrix
Extracellular Matrix - metabolism
Gene Knockdown Techniques
Homeostasis
Models, Biological
Planarians - genetics
Planarians - metabolism
Poly(A)-Binding Protein I - metabolism
Regeneration
RNA, Messenger - genetics
RNA, Messenger - metabolism
Stem cells
Stem Cells and Regeneration
Wound Healing
Poly (A)-binding proteins
Regeneration
Epidermis
Neoblast
Planaria
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Summary:Identifying key cellular events that facilitate stem cell function and tissue organization is critical for understanding the process of regeneration. Planarians are powerful model system to study regeneration and stem cell (neoblast) function. Here, using planaria, we show that the initial events of regeneration, such as epithelialization and epidermal organization are critically regulated by a novel cytoplasmic Poly A binding protein, SMED-PABPC2. Knockdown (KD) of Smed-pabpc2 leads to defects in epidermal lineage specification, disorganization of epidermis and ECM, and deregulated wound healing resulting in the selective failure of neoblast proliferation near the wound region. Polysome profiling suggested epidermal lineage transcripts, including zfp-1, to be translationally regulated by SMED-PABPC2. Together, our results uncover a novel role of SMED-PABPC2 in the maintenance of epidermal and ECM integrity, critical for wound healing, and subsequent processes for regeneration.
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ISSN:0950-1991
1477-9129
1477-9129
DOI:10.1242/dev.152942