In vitro selective suppression of feline myeloid colony formation is attributable to molecularly cloned strain of feline leukemia virus with unique long terminal repeat

• Published in: Research in veterinary science Vol. 78; no. 2; pp. 151 - 154
• Main Authors: Nagashima, N., Hisasue, M., Nishigaki, K., Miyazawa, T., Kano, R., Hasegawa, A.
• Format: Journal Article
• Language: English
• Published: England Elsevier India Pvt Ltd 01.04.2005; Elsevier Limited
• Subjects: acute myeloid leukemia; AML; Animals; Antibiotics; bioassays; Bone marrow; bone marrow cells; Bone Marrow Cells - cytology; Bone Marrow Cells - virology; cat diseases; Cat Diseases - virology; Cats; cell differentiation; Cloning; Cloning, Molecular; colony-stimulating factors; Cytotoxicity; diagnostic techniques; disease models; DNA, Viral - chemistry; DNA, Viral - genetics; Feline leukemia virus; FeLV; hematopoiesis; in vitro studies; Leukemia; Leukemia Virus, Feline - genetics; Leukemia Virus, Feline - pathogenicity; Leukemia, Myeloid, Acute - pathology; Leukemia, Myeloid, Acute - veterinary; Leukemia, Myeloid, Acute - virology; LTR; MDS; molecular cloning; myelodysplastic syndrome; Myelodysplastic Syndromes - veterinary; Myelodysplastic Syndromes - virology; myeloid leukemia; Myeloid Progenitor Cells - cytology; Myeloid Progenitor Cells - virology; pathogenesis; physiological regulation; Polymerase Chain Reaction - veterinary; Retroviridae Infections - veterinary; Retroviridae Infections - virology; Terminal Repeat Sequences; transcription (genetics); transcription factors; Tumor Virus Infections - veterinary; Tumor Virus Infections - virology; Veterinary medicine; AML; Bone marrow; FeLV; LTR; MDS
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2004.07.003

Molecularly cloned feline leukemia virus (FeLV)-clone 33 (C-33), derived from a cat with acute myelocytic leukemia (AML), was examined to assess its relation to the pathogenesis of AML and myelodysplastic syndrome (MDS). To evaluate in vitro pathogenicity of FeLV C-33, bone marrow colony-forming assay was performed on marrow cells infected with FeLV C-33 or an FeLV subgroup A strain (61E, a molecularly cloned strain with minimal pathogenicity). The myeloid colony-forming activity of feline bone marrow mononuclear cells infected with FeLV C-33 was significantly lower than that of cells infected with 61E. This suggests that FeLV C-33 has myeloid lineage-specific pathogenicity for cats, and that FeLV C-33 infection is useful as an experimental model for investigating pathogenesis of MDS and AML.