Comparative sequence analysis of 16S rRNA gene of Pasteurella multocida serogroup B isolates from different animal species

• Published in: Research in veterinary science Vol. 83; no. 1; pp. 1 - 4
• Main Authors: Dey, S., Singh, V.P., Kumar, A.A., Sharma, B., Srivastava, S.K., Singh, Nem
• Format: Journal Article
• Language: English
• Published: England Elsevier India Pvt Ltd 01.08.2007; Elsevier Limited
• Subjects: 16S rRNA; Animal diseases; Animals; Bacterial Vaccines - immunology; Base Sequence; buffaloes; Buffaloes - microbiology; Cattle; Deoxyribonucleic acid; DNA; Epidemiology; Gene amplification; Genes, Bacterial - genetics; genetic distance; genetic polymorphism; genetic variation; Genetics; geographical variation; Goats; Goats - microbiology; Haemorrhagic septicaemia; Hemorrhagic Septicemia - microbiology; Hemorrhagic Septicemia - prevention & control; Hemorrhagic Septicemia - veterinary; Hogs; microbial genetics; Microbiology; molecular epidemiology; molecular sequence data; Molecular weight; nucleotide sequences; Pasteurella multocida; Pasteurella multocida - classification; Pasteurella multocida - genetics; Pasteurella multocida - isolation & purification; pasteurellosis; Phylogenetics; Phylogeny; polymerase chain reaction; ribosomal RNA; RNA, Ribosomal, 16S - genetics; septicemia; Serotype; serotypes; Sheep; Sheep - microbiology; strains; swine; Swine - microbiology; Veterinary medicine; Africa; India; Haemorrhagic septicaemia; 16S rRNA; Serotype; Pasteurella multocida
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2006.10.002

The phylogenetic relationships of five isolates of Pasteurella multocida serotype B:2 belonging to buffalo, cattle, pig, sheep and goat were investigated by comparative sequence analysis of 16S rRNA gene. The 1468 bp fragment of 16S rRNA gene sequence comparison showed that the isolates of cattle (PM75), pig (PM49) and sheep (PM82) shared 99.9% homology with the buffalo isolate (vaccine strain P 52) whereas, the goat isolate (PM86) shared 99.8% homology with the vaccine strain. The 16S rRNA gene sequences of these isolates were also found monophyletic with type B reference strain NCTC 10323 of P. multocida subsp. multocida. The present study indicated the close relationships of haemorrhagic septicaemia causing P. multocida serotype B:2 isolates of buffalo and cattle with other uncommon hosts (pig, sheep and goat).