Novonestmycins A and B, two new 32-membered bioactive macrolides from Streptomyces phytohabitans HBERC-20821

• Published in: Journal of antibiotics Vol. 68; no. 3; pp. 185 - 190
• Main Authors: Wan, Zhongyi, Fang, Wei, Shi, Liqiao, Wang, Kaimei, Zhang, Yani, Zhang, Zhigang, Wu, Zhaoyuan, Yang, Ziwen, Gu, Yucheng
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.03.2015; Japan Antibiotics Research Assoc; Nature Publishing Group
• Subjects: 101/28; 14/5; 38/91; 45/22; 45/77; 631/154/349; 639/638/403/349; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antibiotics; Antibiotics, Antineoplastic - chemistry; Antibiotics, Antineoplastic - isolation & purification; Antibiotics, Antineoplastic - pharmacology; Bacteriology; Biomedical and Life Sciences; Bioorganic Chemistry; Biotechnology & Applied Microbiology; Cell Line, Tumor; Humans; Immunology; Life Sciences; Life Sciences & Biomedicine; Macrolides - chemistry; Macrolides - isolation & purification; Macrolides - pharmacology; Magnetic Resonance Spectroscopy; Medicinal Chemistry; Microbial Sensitivity Tests; Microbiology; Organic Chemistry; original-article; Pharmacology & Pharmacy; Science & Technology; Soil Microbiology; Spectrometry; Streptomyces - metabolism; NOTONESOMYCIN-A
• ISSN: 0021-8820; 1881-1469
• DOI: 10.1038/ja.2014.123

Two new 32-membered macrolide compounds, named Novonestmycins A ( 1 ) and B ( 2 ), were isolated from the soil strain Streptomyces phytohabitans HBERC-20821. Their structures were elucidated by using spectroscopic methods, including 1D, 2D-NMR and MS spectrometry. The two compounds showed strong activities against the phytophathogenic fungi Corynespora cassiicola , Rhizoctonia solani and Septoria nodorum , with MIC values of 0.78, 0.39 and 0.78 μg ml −1 , respectively. In addition, the two compounds exhibited potent inhibitory activities against four different human tumor cell lines as well as one 5-FU-resistant human hepatocellular carcinoma cell line, with IC 50 of 0.15–0.48 μg ml −1 and 0.24–1.34 μg ml −1 , respectively.