The effects of the phospholipase D-antagonist 1-butanol on seedling development and microtubule organisation in Arabidopsis
The organisation of plant microtubules into distinct arrays during the cell cycle requires interactions with partner proteins. Having recently identified a 90-kDa phospholipase D (PLD) that associates with microtubules and the plasma membrane [Gardiner et al. (2001) Plant Cell 13: 2143], we exposed...
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Published in | Plant and cell physiology Vol. 44; no. 7; pp. 687 - 696 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Japan
Oxford University Press
01.07.2003
Oxford Publishing Limited (England) |
Subjects | |
Online Access | Get full text |
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Summary: | The organisation of plant microtubules into distinct arrays during the cell cycle requires interactions with partner proteins. Having recently identified a 90-kDa phospholipase D (PLD) that associates with microtubules and the plasma membrane [Gardiner et al. (2001) Plant Cell 13: 2143], we exposed seeds and young seedlings of Arabidopsis to 1-butanol, a specific inhibitor of PLD-dependent production of the signalling molecule phosphatidic acid (PA). When added to agar growth media, 0.2% 1-butanol strongly inhibited the emergence of the radicle and cotyledons, while 0.4% 1-butanol effectively blocked germination. When normal seedlings were transferred onto media containing 0.2% and 0.4% 1-butanol, the inhibitor retarded root growth by about 40% and 90%, respectively, by reducing cell elongation. Inhibited plants showed significant swelling in the root elongation zone, bulbous or branched root hairs, and modified cotyledon morphology. Confocal immunofluorescence microscopy of root tips revealed that 1-butanol disrupted the organisation of inter- phase cortical microtubules. Butanol isomers that do not inhibit PLD-dependent PA production, 2- and 3-butanol, had no effect on seed germination, seedling growth, or microtubule organisation. We propose that production of PA by PLD may be required for normal microtubule organisation and hence normal growth in Arabidopsis. |
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Bibliography: | F60 F62 2004004029 istex:FF04D449F8DE2B0C477E2450D37C0773B478D7D2 ark:/67375/HXZ-4XCWQWK0-0 local:pcg095 Received December 23, 2002; Accepted May 8, 2003 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0032-0781 1471-9053 1471-9053 |
DOI: | 10.1093/pcp/pcg095 |