Inhibitory effects of chondroitin sulfate prepared from salmon nasal cartilage on fat storage in mice fed a high-fat diet

• Published in: International Journal of Obesity Vol. 24; no. 9; pp. 1131 - 1138
• Main Authors: Han, L.K, Sumiyoshi, M, Takeda, T, Chihara, H, Nishikiori, T, Tsujita, T, Kimura, Y, Okuda, H
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing 01.09.2000; Nature Publishing Group
• Subjects: Absorption; Adipose tissue; Adipose Tissue - metabolism; Administration, Oral; Animals; Biochemistry; Biological and medical sciences; Body weight; brush border membrane vesicles; Cartilage; Cartilage - chemistry; Chondroitin sulfate; Chondroitin Sulfates - pharmacology; Diet; dietary fat; Dietary Fats - administration & dosage; Dose-Response Relationship, Drug; Fatty acids; fatty liver; Female; General and cellular metabolism. Vitamins; glucuronic acid; gum arabic; high fat diet; hyperlipidemia; Hypolipidemic Agents - pharmacology; intestinal absorption; Intestinal Absorption - drug effects; jejunum; Lipase - antagonists & inhibitors; Lipid Metabolism; Lipids; Liver - drug effects; Liver - metabolism; Male; Medical research; Medical sciences; Membranes; Mice; Mice, Inbred ICR; microvilli; nose; Obesity; Obesity - metabolism; Oral administration; palmitic acid; Pancreas - enzymology; Pharmacology. Drug treatments; phosphatidylcholines; Polymers; Rats; Rats, Wistar; Salmon; Small intestine; Sulfates; tissue weight; triacylglycerol lipase; triolein; Japan; Obesity; Enzyme; Rodentia; Nutrition disorder; Gut; Triacylglycerol lipase; Lipids; Esterases; Supplemented diet; Biological activity; Carboxylic ester hydrolases; Vertebrata; Mammalia; Absorption; Enzymatic activity; Mouse; Animal; Fat; Hydrolases; Chondroitin sulfate; Pancreas; Nutritional status
• ISSN: 0307-0565; 1476-5497
• DOI: 10.1038/sj.ijo.0801378

OBJECTIVE: Chondroitin sulfate is an acidic polymer consisting of repeating D-glucuronic acid and D-N-acetylgalactosamine units, and the N-acetylgalactosamine is substituted with the sulfate at either the 4' or 6' position, with approximately one sulfate being present per disaccharide unit. The present study assessed the effects of chondroitin sulfate on the activity of pancreatic lipase and lipid uptake into brush border membrane vesicles of the rat small intestine in vitro, and on the degree of fat storage induced in mice by the oral administration of a high-fat diet for 8 weeks. DESIGN AND MEASUREMENTS: Experiments were carried out to clarify whether or not chondroitin sulfate inhibited pancreatic lipase activity in assay systems using triolein emulsified with phosphatidylcholine or gum arabic. In addition, the effects of chondroitin sulfate on lipid absorption by brush border membrane vesicles were examined. Moreover, mice were fed a high-fat diet and treated with chondroitin sulfate for 8 weeks. RESULTS: Chondroitin sulfate dose-dependently inhibited the pancreatic lipase activity in an assay system using triolein emulsified with phosphatidylcholine. In addition, chondroitin sulfate inhibited the palmitic acid uptake into the brush border membrane vesicles of the rat jejunum. Chondroitin sulfate caused the reduction of body weight and parametrial adipose tissue weight, and prevention of fatty liver and hyperlipidemia in mice fed a high-fat diet. CONCLUSION: The reduction of fat storage and the antihyperlipidemic action of chondroitin sulfate might be due to the inhibition of small intestinal absorption of dietary fat through the inhibition of pancreatic lipase activity and fatty acid uptake through brush border membrane.