Complete genome sequences of cowpea polerovirus 1 and cowpea polerovirus 2 infecting cowpea plants in Burkina Faso

• Published in: Archives of virology Vol. 162; no. 7; pp. 2149 - 2152
• Main Authors: Palanga, Essowè, Martin, Darren P., Galzi, Serge, Zabré, Jean, Bouda, Zakaria, Neya, James Bouma, Sawadogo, Mahamadou, Traore, Oumar, Peterschmitt, Michel, Roumagnac, Philippe, Filloux, Denis
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.07.2017; Springer Nature B.V; Springer Verlag
• Subjects: Annotated Sequence Record; Biomedical and Life Sciences; Biomedicine; Burkina Faso; Genome, Viral; Genomes; Infectious Diseases; Life Sciences; Luteoviridae - genetics; Luteoviridae - isolation & purification; Medical Microbiology; Microbiology and Parasitology; Open Reading Frames; Phylogeny; Plant Diseases - virology; Polymerase chain reaction; Recombination; RNA, Viral - genetics; Vigna - virology; Virology; Ribosomal Frameshift; Beckman Coulter Genomic; Species Demarcation Criterion; Unique Recombination Event; Accession Number NC008249; burkina faso; virus de la mosaïque de la niebe; séquence de gènes; pathologie végétale; polerovirus; virologie végétale
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-017-3327-z

The full-length genome sequences of two novel poleroviruses found infecting cowpea plants, cowpea polerovirus 1 (CPPV1) and cowpea polerovirus 2 (CPPV2), were determined using overlapping RT-PCR and RACE-PCR. Whereas the 5845-nt CPPV1 genome was most similar to chickpea chlorotic stunt virus (73% identity), the 5945-nt CPPV2 genome was most similar to phasey bean mild yellow virus (86% identity). The CPPV1 and CPPV2 genomes both have a typical polerovirus genome organization. Phylogenetic analysis of the inferred P1-P2 and P3 amino acid sequences confirmed that CPPV1 and CPPV2 are indeed poleroviruses. Four apparently unique recombination events were detected within a dataset of 12 full polerovirus genome sequences, including two events in the CPPV2 genome. Based on the current species demarcation criteria for the family Luteoviridae , we tentatively propose that CPPV1 and CPPV2 should be considered members of novel polerovirus species.