Grafting of polylysine with polyethylenoxide prevents demixing of O-pyromellitylgramicidin in lipid membranes

• Published in: Biochimica et biophysica acta Vol. 1758; no. 10; pp. 1685 - 1695
• Main Authors: Pashkovskaya, A.A., Lukashev, E.P., Antonov, P.E., Finogenova, O.A., Ermakov, Yu.A., Melik-Nubarov, N.S., Antonenko, Yu.N.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2006
• Subjects: Cluster; Domain; Gramicidin - analogs & derivatives; Gramicidin - chemistry; Ion channel; Lipid Bilayers - chemistry; Liposomes - chemistry; Membrane Lipids - chemistry; Membranes, Artificial; Phospholipid membrane; Polyethylene Glycols - chemistry; Polylysine - chemistry; Polymer; BLM; OPg; PLL–PEO20000; EL; CL; Cluster; Polymer; DPhPC; Ion channel; Phospholipid membrane; PLL; PLL–PEO4000; gA; PEO; Domain; AlPcS 3; DPPC
• ISSN: 0005-2736; 0006-3002; 1879-2642
• DOI: 10.1016/j.bbamem.2006.06.011

Both natural and synthetic polycations can induce demixing of negatively charged components in artificial and possibly in natural membranes. This process can result in formation of clusters (binding of several components to a polycation chain) and/or domains (aggregation of clusters and formation of a separate phase enriched in some particular component). In order to distinguish between these two phenomena, a model lipid membrane system containing ion channels, formed by a negatively charged peptide, O-pyromellitylgramicidin, and polycations of different structures was used. Microelectrophoresis of liposomes, changes in boundary potential of planar bilayers, the shape of compression curves and potentials of lipid and lipid/peptide monolayers were used to monitor the electrostatic factors in polymer adsorption to the membrane and peptide–polymer interactions. The synthesized PEO-grafted polylysine, PLL–PEO20000, did not induce peptide demixing monitored by stabilization of the gramicidin channels, in contrast to parent polylysine (PLL). Both polymers were shown to bind effectively to negatively charged liposomes and lipid monolayers, suggesting that the ineffectiveness of PLL–PEO20000 was not due to reduction of its binding. It was hypothesized that PLL–PEO20000 could not induce domain formation due to steric hindrance of long PEO chains preventing lateral fusion of clusters. Another copolymer, PLL–PEO4000, having four PEO chains of 4000 Da, exhibited intermediate effect between PLL and PLL–PEO20000, which shows the importance of the copolymer architecture for the effect on the lateral distribution of OPg channels. The model system can be relevant to regulation of lateral organization of ion channels and other components in natural membrane systems.