Changes of c-fos, Malondialdehyde and Lactate in Brain Tissue after Global Cerebral Ischemia under Different Brain Temperatures

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 34; no. 3; pp. 354 - 358
• Main Author: 张洪 黎黎 许国英 梅元武 章军建 慕容慎行 孙圣刚 童萼塘
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.06.2014; Department of Neurology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China%Department of Neurology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China%Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
• Subjects: Animals; Body Temperature; Brain - blood supply; Brain - metabolism; Brain - physiopathology; Brain Ischemia - metabolism; Brain Ischemia - physiopathology; c-fos蛋白; Cerebral Cortex - blood supply; Cerebral Cortex - metabolism; Cerebral Cortex - physiopathology; Hippocampus - blood supply; Hippocampus - metabolism; Hippocampus - physiopathology; Immunochemistry; Lactic Acid - metabolism; Male; Malondialdehyde - metabolism; MDA含量; Medicine; Medicine & Public Health; Proto-Oncogene Proteins c-bcl-2 - metabolism; Proto-Oncogene Proteins c-fos - metabolism; Rats; Rats, Sprague-Dawley; Reperfusion Injury - metabolism; Reperfusion Injury - physiopathology; Spectrophotometry; Temperature; Time Factors; Tumor Suppressor Protein p53 - metabolism; 丙二醛; 乳酸; 常温条件; 温度条件; 脑组织; 脑缺血再灌注; mild hypothermia; c-fos; hyperthermia; malondialdehyde; lactate; cerebral ischemia
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-014-1282-4

Summary： Under global cerebral ischemia, the effect of different brain temperature on cerebral ischemic injury was studied. Male Sprague-Dawley rats were divided into normothermic （37-38℃） ischemia, mild hypothermic （31 32℃） ischemia, hyperthermic （41-42℃） ischemia and sham-operated groups. Global cerebral ischemia was established using the Pulsinelli four-vessel occlusion model and brain temperature was maintained at defined level for 60 min after 20omin ischemia. The expression of c-fos protein and the levels of malondialdehyde （MDA） and lactate in brain regions were detected by immunochemistry and spectrophotometrical methods, respectively. C-fos positive neurons were found in the hippocampus and cerebral cortex after cerebral ischemia reperfusion. Mild hypothermia increased the expression of c-fos protein in both areas, whereas hyperthermia decreased the expression of c-los protein in the hippocampus at 24 h reperfusion, and the cerebral cortex at 48 h reperfusion when compared to normothermic conditions. In normothermic, mild hypothermic and hyperthermic ischemia groups, the levels of MDA and lactate in brain tissue were increased at 24, 48 and 72 h reperfusion fol- lowing 20-min ischemia as compared with the sham-operated group （P〈0.01）. The levels of MDA and lactate in mild hypothermic group were significantly lower than those in normothermic group （P〈0.01）. It is suggested that brain temperature influences the translation of the immunoreactive protein product of c-fos after global cerebral ischemia, and MDA and lactate are also affected by hypothermia and hyperthermia.