Arabidopsis CAMTA family proteins enhance V-PPase expression in pollen

• Published in: Plant and cell physiology Vol. 44; no. 10; pp. 975 - 981
• Main Authors: Mitsuda, N. (Kyoto Univ. (Japan)), Isono, T, Sato, M.H
• Format: Journal Article
• Language: English
• Published: Japan Oxford University Press 01.10.2003; Oxford Publishing Limited (England)
• Subjects: ARABIDOPSIS; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Base Sequence; CAM-binding transcriptional activator; CAMTA; DNA Primers; DNA, Complementary - genetics; GENE EXPRESSION; Gene Library; GFP; Glucuronidase - genetics; Glucuronidase - metabolism; green fluorescent protein; GUS; Keywords: AtCAMTA — AVP1 — Gene expression — Pollen — Transcription factor; Plants, Genetically Modified; POLLEN; Polymerase Chain Reaction; Pyrophosphatases - genetics; Recombinant Proteins - metabolism; Restriction Mapping; RNA, Messenger - genetics; TRANSCRIPTION FACTORS; V-PPase; vacuolar H+-translocating inorganic pyrophosphatase; β-glucronidase
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/pcp/pcg137

The pollen-specific cis-acting region of the AVP1 gene is involved in the expression of the Arabidopsis V-PPase gene during pollen development. We isolate AtCAMTA5, which binds to the 38-bp pollen-specific cis-acting region, by one-hybrid screening using the cis-acting region as a probe. The green fluorescent protein-fused AtCAMTA5 is specifically localized to the nucleus in Arabidopsis suspen-sion cultured cells. The promoter-v-glucuronidase reporter experiment shows the expression not only of AtCAMTA5 but also of AtCAMTA1 in pollen. In particular, AtCAMTA1 is specifically expressed in pollen. Both the one-hybrid analysis in the reporter yeast and in vivo tran-sient effector-reporter analysis in Arabidopsis suspension cultured cells revealed that AtCAMTA1 could regulate gene expression depending on the CGCG-box within the 38-bp pollen-specific cis-acting region. These results indicate that AtCAMTA1 as well as AtCAMTA5 possibly enhance AVP1 expression in pollen,