Xylo-oligosaccharides ameliorate high cholesterol diet induced hypercholesterolemia and modulate sterol excretion and gut microbiota in hamsters

• Published in: Journal of functional foods Vol. 77; p. 104334
• Main Authors: Abdulaziz Abbod Abdo, Abdullah, Zhang, Chengnan, Lin, Yuling, Liang, Xin, Kaddour, Benariba, Wu, Qiuhua, Li, Xiuting, Fan, Guangsen, Yang, Ran, Teng, Chao, Xu, Youqiang, Li, Weiwei
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.02.2021; Elsevier
• Subjects: Cholesterol-lowering activity; Gut microbiota; Neutral and acidic sterols; Short chain fatty acids; Xylo-oligosaccharides; Gut microbiota; UDCA; Short chain fatty acids; CYP7A1; GCDCA; Xylo-oligosaccharides; Cholesterol-lowering activity; Alpha- MA; GDCA; X2; X3; Neutral and acidic sterols; X4; X5; CA; CDCA; ASS; DCA; GCA; HCD; TDCA; LCA; LCD; TLCA; TCA; Beta- MA; SCFAs; HDCA; DHCA; XOS
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2020.104334

[Display omitted] •Xylo-oligosaccharides reduced plasma total cholesterol levels.•Xylo-oligosaccharides increased fecal outputs of neutral and acidic sterols.•Xylo-oligosaccharides increased fecal outputs of short chain fatty acids.•Xylo-oligosaccharides influenced gut microbiota composition. The present study investigated the cholesterol-lowering activity of xylo- oligosaccharides and its associated underlying mechanisms in hamsters. Twenty-four hamsters were randomly divided into three groups and fed one of three diets, namely a low cholesterol diet, a high cholesterol diet (HCD), and an HCD diet with supplementation of 5% xylo-oligosaccharides for 6 weeks. The changes in gut microbiota, fecal neutral and acidic sterols were examined. Results exhibited that xylo- oligosaccharides could significantly reduce plasma total cholesterol, non-high-density lipoprotein cholesterol and total triacylglycerol by 11.24%, 24.89% and 38.72%, respectively (p < 0.05). Such benefits were associated with an increase in fecal outputs of neutral and acidic sterols as well as SCFAs. Furthermore, dietary supplementation with xylo-oligosaccharides could change the composition of gut microbiota. It was therefore concluded that xylo-oligosaccharides supplementation could reduce plasma cholesterol levels, enhance the excretion of neutral and acidic sterols, and promote the production of SCFAs via changing the gut microbiota composition.