Effect of bone morphogenetic protein-12 gene transfer on mesenchymal progenitor cells

• Published in: Clinical orthopaedics and related research no. 369; p. 333
• Main Authors: Lou, J, Tu, Y, Ludwig, F J, Zhang, J, Manske, P R
• Format: Journal Article
• Language: English
• Published: United States 01.12.1999
• Subjects: Adenoviridae - genetics; Alkaline Phosphatase - analysis; Animals; Blotting, Western - methods; Bone Morphogenetic Proteins - genetics; Bone Morphogenetic Proteins - pharmacology; Cartilage - drug effects; Cell Differentiation - genetics; Cells, Cultured; Gene Expression Regulation - genetics; Gene Transfer Techniques; Hindlimb; Humans; Mesoderm - cytology; Mesoderm - enzymology; Mice; Mice, Inbred C3H; Recombination, Genetic; Stem Cells - cytology; Stem Cells - enzymology; Tendons - drug effects
• ISSN: 0009-921X
• DOI: 10.1097/00003086-199912000-00035

Recombinant adenovirus mediated human bone morphogenetic protein-12 gene transfer induced tendon and cartilage-like tissue formation in vivo. The recombinant adenovirus with the human bone morphogenetic protein-12 gene was constructed, and mature human bone morphogenetic protein-12 expression mediated by adenovirus gene transfer was detected by specific antibody. Unlike bone morphogenetic protein-2 gene transfer, bone morphogenetic protein-12 gene transferred mesenchymal progenitor cell line C3H 10T1/2 showed no change of alkaline phosphatase activity, which is the mark of cell differentiation into osteoblastic phenotype. Injection of bone morphogenetic protein-12 gene transferred C3H 10T1/2 cells into nude mice thigh muscles induced tendon and cartilage-like tissue formation. The results indicate bone morphogenetic protein-12 has different effects on mesenchymal progenitor cell differentiation, and it may influence the cell differentiation into a nonosteoblast lineage.