The Interaction of Copper-Bipyridyl Complex with DNA and Cleavage to DNA

The interaction of a copper-bipyridyl (bpy) complex with CT-DNA was investigated by voltammetry, absorption and fluorescence spectrophotometry. The binding constant of the Cu(bpy)22+ complex interacting with DNA was 3.24 × 104 L/mol and the ability binding of Cu(bpy)22+ to DNA was 1.3-times as large...

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Published inAnalytical Sciences Vol. 20; no. 8; pp. 1127 - 1130
Main Authors YANG, Zhou-Sheng, WANG, Yan-Ling, ZHAO, Guang-Chao
Format Journal Article
LanguageEnglish
Published Japan The Japan Society for Analytical Chemistry 01.08.2004
Japan Science and Technology Agency
Subjects
2,2'-Dipyridyl - chemistry
Chromatography, High Pressure Liquid
Copper - chemistry
DNA - chemistry
DNA Damage
Organometallic Compounds - chemistry
Spectrometry, Fluorescence
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Summary:The interaction of a copper-bipyridyl (bpy) complex with CT-DNA was investigated by voltammetry, absorption and fluorescence spectrophotometry. The binding constant of the Cu(bpy)22+ complex interacting with DNA was 3.24 × 104 L/mol and the ability binding of Cu(bpy)22+ to DNA was 1.3-times as large as that of Cu(bpy)2+ to DNA. DNA could be efficiently cleaved by a potential-modulated method in the presence of the Cu(bpy)22+ complex. The fragments of the cleaved DNA were separated by high-performance liquid chromatography (HPLC). The experimental results revealed that the proposed method for DNA cleavage is highly efficient.
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ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.20.1127