The Mouse ZFH-4 Protein Contains Four Homeodomains and Twenty-Two Zinc Fingers

We isolated the mouse zfh-4 cDNA which is 12 kb long and capable of encoding a 3,550-amino acid protein containing four homeodomains and 22 zinc fingers including two pseudo zinc finger motifs. The mouse ZFH-4 is 51% homologous to the mouse ATBF1 and 23% to the Drosophila ZFH-2. The homeodomain and...

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Published inBiochemical and biophysical research communications Vol. 273; no. 2; pp. 686 - 693
Main Authors Sakata, Nobuo, Hemmi, Kazunori, Kawaguchi, Makoto, Miura, Yutaka, Noguchi, Shigeru, Ma, Dongping, Sasahara, Masakiyo, Kato, Taiji, Hori, Makoto, Tamaoki, Taiki
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 05.07.2000
Subjects
Amino Acid Motifs - genetics
Amino Acid Sequence
Animals
ATBF1
Base Sequence
Cloning, Molecular
DNA Primers - genetics
DNA, Complementary - genetics
DNA, Complementary - isolation & purification
Exons
Gene Expression
homeodomain
Homeodomain Proteins - chemistry
Homeodomain Proteins - genetics
Introns
Mice
Molecular Sequence Data
Restriction Mapping
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Homology, Amino Acid
transcription factor
ZFH-2
ZFH-4
zinc finger
Zinc Fingers - genetics
transcription factor
ATBF1
ZFH-2
zinc finger
homeodomain
ZFH-4
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Summary:We isolated the mouse zfh-4 cDNA which is 12 kb long and capable of encoding a 3,550-amino acid protein containing four homeodomains and 22 zinc fingers including two pseudo zinc finger motifs. The mouse ZFH-4 is 51% homologous to the mouse ATBF1 and 23% to the Drosophila ZFH-2. The homeodomain and zinc finger regions are highly conserved between ZFH-4 and ATBF1 except that one zinc finger is missing in ZFH-4. Analysis of partial genomic sequences showed that the mouse zfh-4 and ATBF1 genes are similar in exon-intron organization. RT-PCR analysis of zfh-4 transcripts in adult mouse tissues showed that zfh-4 expression was low but reproducibly detectable in brain, heart, lung and muscle. In these mouse tissues, ATBF1 transcripts were poorly amplified by PCR under the conditions where zfh-4 transcripts were amplified, suggesting that the expression of zfh-4 mRNA is higher than that of ATBF1 mRNA. Other comparative analysis suggests functional similarities and dissimilarities between ZFH-4 and ATBF1.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2000.2990