The influence of reduced glutathione in fertilization medium on the fertility of in vitro–matured C57BL/6 mouse oocytes

It is well known that IVM oocytes show a decreased potential for fertility and development compared with in vivo–matured oocytes. In this study, we added reduced glutathione (GSH) to the fertilization medium during IVF to investigate its effect on the fertility and early embryo development of IVM oo...

Full description

Saved in:
Bibliographic Details
Published inTheriogenology Vol. 80; no. 5; pp. 421 - 426
Main Authors Ishizuka, Y., Nishimura, M., Matsumoto, K., Miyashita, M., Takeo, T., Nakagata, N., Hosoi, Y., Anzai, M.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.09.2013
Subjects
Animals
C57BL/6J
Cryopreservation
Culture Media
drilling
embryogenesis
Fertility
Fertilization in Vitro - methods
Frozen/thawed sperm
glutathione
Glutathione - pharmacology
in vitro fertilization
In Vitro Oocyte Maturation Techniques - methods
In vitro fertilization
In vitro maturation
Male
Mice
Mice, Inbred C57BL
oocytes
Oocytes - metabolism
progeny
Spermatozoa
Frozen/thawed sperm
C57BL/6J
In vitro fertilization
In vitro maturation
Online AccessGet full text

Cover

More Information
Summary:It is well known that IVM oocytes show a decreased potential for fertility and development compared with in vivo–matured oocytes. In this study, we added reduced glutathione (GSH) to the fertilization medium during IVF to investigate its effect on the fertility and early embryo development of IVM oocytes. The fertilization rate for IVM oocytes and fresh sperm increased with the addition of GSH (0, 1.0, and 2.0 mM: 51%, 76%, and 70%). Moreover, the addition of GSH to the fertilization medium also improved the developmental potential compared with the control sample (0 mM). In addition, we performed IVF using IVM oocytes and frozen/thawed sperm that had been cryopreserved in a mouse bank. Results indicated a marked increase in the fertilization rate when 1.0 mM GSH was added to the fertilization medium compared with when no GSM was used (0.0 mM GSH: 2% (3/195); 1.0 mM GSH: 33% (156/468)). Furthermore, the fertilization rate improved dramatically via zona drilling using laser equipment (52%: 267/516), whereas normal offspring were obtainsed after transferring embryos created via IVF using IVM oocytes and frozen/thawed sperm. This is the first report in which offspring have been obtained via IVF using IVM oocytes and frozen/thawed sperm.
Bibliography:http://dx.doi.org/10.1016/j.theriogenology.2013.07.002
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-1
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2013.07.002