Osteopontin Promotes Expression of Matrix Metalloproteinase 13 through NF-κB Signaling in Osteoarthritis

• Published in: BioMed research international Vol. 2016; no. 2016; pp. 1 - 8
• Main Authors: Luo, Wei, Lei, Guanghua, Gao, Shuguang, Li, Liangjun, Tu, Min, Zeng, Chao, Deng, Zhenhan, Wang, Hua, Jiang, Wei, Li, Yusheng, Xiao, Wenfeng
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2016
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2016/6345656

Osteopontin (OPN) is associated with the severity and progression of osteoarthritis (OA); however, the mechanism of OPN in the pathogenesis of OA is unknown. In this study, we found that OA patients had higher abundance of OPN and matrix metalloproteinase 13 (MMP13). In chondrocytes, we showed that OPN promoted the production of MMP13 and activation of NF- κ B pathway by increasing the abundance of p65 and phosphorylated p65 and translocation of p65 protein from cytoplasm to nucleus. Notably, inhibition of NF- κ B pathway by inhibitor suppressed the production of MMP13 induced by OPN treatment. In conclusion, OPN induces production of MMP13 through activation of NF- κ B pathway.