Functional status of cells from lymphoid and myeloid tissues in mice with severe combined immunodeficiency disease

• Published in: The Journal of immunology (1950) Vol. 132; no. 4; pp. 1804 - 1808
• Main Authors: Dorshkind, K, Keller, GM, Phillips, RA, Miller, RG, Bosma, GC, O'Toole, M, Bosma, MJ
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 01.04.1984; American Association of Immunologists
• Subjects: Animals; B-Lymphocytes - cytology; B-Lymphocytes - immunology; Biological and medical sciences; Bone Marrow - immunology; Bone Marrow Cells; Cell Differentiation; Colony-Forming Units Assay; Cytotoxicity, Immunologic; Experimental and animal immunopathology. Animal models; Hematopoietic Stem Cells - cytology; Immunologic Deficiency Syndromes - blood; Immunologic Deficiency Syndromes - immunology; Immunopathology; Lymphocyte Activation; Lymphoid Tissue - cytology; Lymphoid Tissue - immunology; Medical sciences; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Mutant Strains; T-Lymphocytes - cytology; T-Lymphocytes - immunology; Animal model; Vertebrata; Lymphoid cell; Mammalia; Rodentia; Combined immune deficiency; Myeloid cell
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.132.4.1804

Cells from mice with severe combined immunodeficiency disease (SCID) were tested in assays that measure myeloid and lymphoid function. Results showed that C.B-17 scid and their normal counterparts (C.B-17) have similar levels of spleen colony-forming units. The frequency of in vitro myeloid colony-forming units in C.B-17 scid spleen is elevated, but the absolute number of colony-forming units in C.B-17 scid and C.B-17 spleen is similar. The absolute number of bone marrow colony-forming units in C.B-17 scid and C.B-17 mice is comparable. Cells from C.B-17 scid spleen are consistently negative in all tests of B and T cell function. C.B-17 scid splenocytes fail to proliferate in response to T and B cell mitogens or to allogeneic lymphocytes in a one-way MLR; C.B-17 scid cells do serve as stimulators in MLR. B lymphocyte colony-forming units are absent, as are cytotoxic lymphocyte precursors and cells that can generate T cell colonies with cytotoxic progenitors. The microenvironment of the C.B-17 scid mouse is conducive to lymphocyte differentiation, because functional B and T cells are easily detectable in mice reconstituted with normal bone marrow cells. The results of this study indicate that scid specifically impairs the differentiation of stem cells into mature lymphocytes; myeloid cell differentiation is not affected.